Table 1.
Effect of chromanol 293B on motility parameters during human sperm capacitation. Values represent the means ± SEM of 5 experiments.
DMSO | 100μM Chromanol 293B | 200μM Chromanol 293B | |
---|---|---|---|
Motility (%) | 67.06 ± 1.33 | 63.26 ± 0.80* | 34.30 ± 1.24* |
Progressive motility (%) | 46.76 ± 1.13 | 42.89 ± 0.89* | 21.90 ± 0.66* |
VAP (μm/s) | 81.81 ± 1.64 | 78.52 ± 1.40 | 70.17 ± 1.07* |
VSL (μm/s) | 72.25 ± 1.62 | 69.01 ± 1.33 | 59.46 ± 0.95* |
VCL (μm/s) | 130.33 ± 2.66 | 126.99 ± 2.78 | 118.95 ± 1.91* |
ALH (μm) | 5.65 ± 0.14 | 5.52 ± 0.14 | 5.91 ± 0.07 |
BCF (Hz) | 31.48 ± 0.29 | 32.01 ± 0.31 | 25.67 ± 0.29* |
STR (%) | 85.12 ± 0.42 | 84.50 ± 0.38 | 81.90 ± 0.31* |
LIN (%) | 54.06 ± 0.44 | 53.10 ± 0.38 | 49.30 ± 0.45* |
Hyperactivation(%) | 7.00 ± 0.54 | 7.01 ± 0.82 | 7.70 ± 0.68 |
p<0.05.
VAP, average path velocity; VSL, straight line velocity; VCL, curvilinear velocity; ALH, amplitude of lateral head displacement; BCF, beat-cross frequency; STR, straightness (VSL/VAP multiplied by 100); LIN, linearity (VSL/VCL multiplied by 100); Hyperactivation, VCL≥150μm/s, ALH≥7.0μm, and LIN≤50%.