FIGURE 2.

HGF induced MYCN transcription requires an intact E2F site. (A) A MYCN promoter-luciferase construct was transfected into HepG2 cells followed by treatment with HGF. The cells were harvested at indicated time points and luciferase activities were normalized by GFP fluorescence and protein concentration. (B) IPA analysis of upstream regulators of MYCN. (C) Primary murine hepatocytes were transfected with indicated siRNAs. Knockdown efficiencies were verified by Western. (D,E) Primary murine hepatocytes were transfected with indicated siRNAs followed by treatment with HGF for 24 h. MYCN expression was examined by qPCR and Western. (F) WT or mutant MYCN promoter-luciferase construct was transfected into HepG2 cells followed by treatment with HGF for 24 h. Luciferase activities were normalized by GFP fluorescence and protein concentration. Data are expressed as mean ± standard deviation (SD). *p<0.05 (one-way ANOVA with post hoc Scheffe test).