FIG. 2.

Caspase 8 is processed in target cells following the addition of whole CTL or anti-Fas antibody. (A) Jurkat cells were incubated with whole hCTL at an effector-to-target ratio of 2:1. Cell lysates were generated at the times indicated and immunoblotted for caspase 8 activation. (B) Jurkat cells were treated with 250 ng of anti-Fas antibody per ml for 0, 4, 8, and 20 h, and caspase 8 activation was assessed by Western blotting analysis. (C) Jurkat cells were incubated with whole hCTL at an effector-to-target ratio of 2:1. Cell lysates were generated at the times indicated and immunoblotted for caspase 3 activation.