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. 2021 Oct 22;12:722564. doi: 10.3389/fgene.2021.722564

FIGURE 5.

FIGURE 5

miR-139-5p overexpression could inhibit the osteogenic differentiation of VICs. (A) The expression levels of miR-139-5p in negative control (NC) mimics and miR-139-5p mimics (miR-139 mimic) transfected VICs were accessed by qRT-PCR and normalized with U6 (n = 4 samples from independent wells in each group). Unpaired Student’s t-test, **p < 0.01. (B) Alizarin red staining was used to access the matrix calcium deposition in NC mimics and miR-139-5p mimics transfected VICs at 15 days after OM induction and the right graph was the percentage of Alizarin red positive area per field (n = 3 wells in each group and three to four fields per well were counted, the scale bar = 200 μm). Unpaired Student’s t-test, **p < 0.01. (C–E) The expression levels of BGLAP (C), ALPP (D) and RUNX2 (E) in NC mimics and miR-139-5p mimics transfected VICs were accessed by qRT-PCR and normalized with GAPDH (n = 4 samples from independent wells in each group). (F,G) The expression levels of FZD4 (F) and CTNNB1 (G) in NC mimics and miR-139-5p mimics transfected VICs were accessed by qRT-PCR and normalized with GAPDH (n = 4 samples from independent wells in each group). One-way ANOVA, *p < 0.05, **p < 0.01.