Figure 3. Effect of WYE on 3D tumor spheroids. Cell viability and morphological changes in MDA-MB-231 tumor spheroids with increasing concentration of WYE are shown. The data represent basic changes in spheroid structure (main image; black and white), with fluorescent FDA viable cell staining (green) in the lower left section of each main image. (A) Control, WYE Treatment (B) 4.62 μg/ml, (C) 9.25 μg/ml, (D) 18.5 μg/ml, (E) 37 μg/ml, (F) 74 μg/ml, (G) 148 μg/ml, (H) 296 μg/ml.
