Table 2. Experimental design for the primary immunization of mice.
| Group | No. of mice/group | Antigen | Dosage/vol | Adjuvant | Route |
|---|---|---|---|---|---|
| Negative control | 10 | Phosphate-buffered saline (PBS) | 100 μl | Freund’s complete | Subcutaneous |
| Inactivated group | 10 | Inactivated straina | 4.0 × 109 CFU/100 μl | Freund’s complete | Subcutaneous |
| Recombinant protein LpxC | 10 | LpxC | 100 μg/100 μl | Freund’s complete | Subcutaneous |
| Recombinant protein GmhA | 10 | GmhA | 100 μg/100 μl | Freund’s complete | Subcutaneous |
| LpxC+ GmhA mixed protein | 10 | LpxC+ GmhA | 100 μg/100 μlb | Freund’s complete | Subcutaneous |
a CY1201 strain (Serotype 13) inactivated with formaldehyde. b The mice from LpxC+GmhA mixed protein were immunized with 50 μg of LpxC and 50 μg of GmhA.