Table 2. Oligonucleotide sequences used for semi-qRT-PCR for HVT080, HVT085, HVT012, HVT060, HVT052, HVT053, and chicken GAPDH genes.
| Molecules | Primers (5′ to 3′) |
|---|---|
| HVT080 | ATGGCATCCCCTTCAGAATATGACC |
| TGGAGAAGGGACGGTCTACTGAAGA | |
| HVT085 | ATGGATGGGAAAACTACACACGTGA |
| ACCTAGCATATACATATCAAACAAA | |
| HVT012 | ATGGCTCGTAGTTCAACTGACAGGTT |
| TGTAGTGCCAGTGATTATACAGTCC | |
| HVT060 | ATGATGGATCAAAGCCTTAAGTCTG |
| GGCAGAATTCAATTCTTAGACTCTC | |
| HVT052 | ATGGTTTCCAACATGCGCGTTCTAC |
| TATATCAGGTATTTTGTGAGAAGAG | |
| HVT053 | ATGATGTCGCCCACCCCTGAAGATG |
| TTGCATTTTTCCCGGCCACGCGCAT | |
| Chicken GAPDH | GGTGGTGCTAAGCGTGTTAT |
| ACCTCTGTCATCTCTCCACA | |