TABLE 1.
The Plackett–Burman design with the actual (mg/g CFK) and codified values of the tested medium components of B. paramycoides ZW-5 and the corresponding keratinolytic activity and amino acids.
| Trial | K2HPO4 |
KH2PO4 |
NaCl |
NH4Cl |
MgSO4 |
CaCl2 |
AA (μg/g CFK) ± SD |
|||||||
| Actual | Coded | Actual | Coded | Actual | Coded | Actual | Coded | Actual | Coded | Actual | Coded | Response | Predicted | |
| 1 | 1.8 | 1 | 0.4 | −1 | 0.7 | 1 | 0.5 | −1 | 0.05 | −1 | 0.005 | −1 | 490.014.0 | 370.3 |
| 2 | 1.8 | 1 | 1.0 | 1 | 0.3 | −1 | 1.5 | 1 | 0.05 | −1 | 0.005 | −1 | 760.08.5 | 762.7 |
| 3 | 1.0 | −1 | 1.0 | 1 | 0.7 | 1 | 0.5 | −1 | 0.15 | 1 | 0.005 | −1 | 104.313.8 | 125.7 |
| 4 | 1.8 | 1 | 0.4 | −1 | 0.7 | 1 | 1.5 | 1 | 0.05 | −1 | 0.015 | 1 | 1,209.116.5 | 1305.5 |
| 5 | 1.8 | 1 | 1.0 | 1 | 0.3 | −1 | 1.5 | 1 | 0.15 | 1 | 0.005 | −1 | 712.112.6 | 709.3 |
| 6 | 1.8 | 1 | 1.0 | 1 | 0.7 | 1 | 0.5 | −1 | 0.15 | 1 | 0.015 | 1 | 93.36.5 | 159.4 |
| 7 | 1.0 | −1 | 1.0 | 1 | 0.7 | 1 | 1.5 | 1 | 0.05 | −1 | 0.015 | 1 | 1,168.717.5 | 1114.2 |
| 8 | 1.0 | −1 | 0.4 | −1 | 0.7 | 1 | 1.5 | 1 | 0.15 | 1 | 0.005 | −1 | 1,228.021.6 | 1218.3 |
| 9 | 1.0 | −1 | 0.4 | −1 | 0.3 | −1 | 1.5 | 1 | 0.15 | 1 | 0.015 | 1 | 1,262.015.3 | 1229.8 |
| 10 | 1.8 | 1 | 0.4 | −1 | 0.3 | −1 | 0.5 | −1 | 0.15 | 1 | 0.015 | 1 | 371.320.8 | 328.4 |
| 11 | 1.0 | −1 | 1.0 | 1 | 0.3 | −1 | 0.5 | −1 | 0.05 | −1 | 0.015 | 1 | 223.69.5 | 190.5 |
| 12 | 1.0 | −1 | 0.4 | −1 | 0.3 | −1 | 0.5 | −1 | 0.05 | −1 | 0.005 | −1 | 240.08.8 | 348.1 |