TABLE 2.
The RCCD matrix of the independent factors, showing the experimental data of amino acids production, and the corresponding predicted values obtained from RCCD and ANN models.
| Run | KH2PO4 (mg/g CFP) | NH4Cl (mg/g CFP) | Incubation time (day) | Amino acids (μg/g CFK) |
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|
|
Observed | RCCD |
ANN |
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| Coded (X1) | Actual | Coded (X2) | Actual | Coded (X3) | Actual | Predicted | Residual | Predicted | Residual | ||
| 1* | −1 | 0.400 | −1 | 1.00 | −1 | 7.00 | 1,102.30.9 | 1,127.2 | –24.9 | 1,080.3 | 22.0 |
| 2 | 1 | 0.700 | −1 | 1.00 | −1 | 7.00 | 1,069.42.2 | 1,035.9 | 33.5 | 1,069.0 | 0.4 |
| 3 | −1 | 0.400 | 1 | 2.00 | −1 | 7.00 | 974.91.4 | 947.2 | 27.7 | 980.2 | –5.3 |
| 4* | 1 | 0.700 | 1 | 2.00 | −1 | 7.00 | 637.62.3 | 658.0 | –20.4 | 647.9 | –10.3 |
| 5* | −1 | 0.400 | −1 | 1.00 | 1 | 14.00 | 1,111.11.9 | 1,136.2 | –25.1 | 1,149.1 | –38.0 |
| 6* | 1 | 0.700 | −1 | 1.00 | 1 | 14.00 | 1,098.41.6 | 1,137.7 | –39.3 | 1,091.6 | 6.8 |
| 7* | −1 | 0.400 | 1 | 2.00 | 1 | 14.00 | 1,189.35.1 | 1,225.5 | –36.2 | 1,189.4 | –0.1 |
| 8 | 1 | 0.700 | 1 | 2.00 | 1 | 14.00 | 1,059.94.6 | 1,029.3 | 30.6 | 1,055.6 | 4.3 |
| 9* | −1.682 | 0.298 | 0 | 1.50 | 0 | 10.50 | 1,040.41.6 | 1,025.1 | 15.3 | 1,039.9 | 0.5 |
| 10* | 1.682 | 0.802 | 0 | 1.50 | 0 | 10.50 | 791.02.5 | 783.3 | 7.7 | 762.1 | 28.9 |
| 11 | 0 | 0.550 | −1.682 | 0.66 | 0 | 10.50 | 1,285.12.9 | 1,280.7 | 4.4 | 1,298.1 | –13.0 |
| 12 | 0 | 0.550 | 1.682 | 2.34 | 0 | 10.50 | 1,019.41.2 | 1,038.1 | –18.7 | 1,030.5 | –11.1 |
| 13* | 0 | 0.550 | 0 | 1.50 | −1.682 | 4.61 | 961.61.5 | 989.0 | –27.4 | 994.0 | –32.4 |
| 14* | 0 | 0.550 | 0 | 1.50 | 1.682 | 16.39 | 1,312.13.0 | 1,308.7 | 3.4 | 1,337.8 | –25.7 |
| 15* | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,601.52.1 | 1,626.0 | –24.5 | 1,626.0 | –24.5 |
| 16 | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,541.73.1 | 1,626.0 | –84.3 | 1,626.0 | –84.3 |
| 17* | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,649.45.1 | 1,626.0 | 23.4 | 1,626.0 | 23.4 |
| 18 | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,709.93.6 | 1,626.0 | 83.9 | 1,626.0 | 83.9 |
| 19 | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,602.33.1 | 1,626.0 | –23.7 | 1,626.0 | –23.7 |
| 20* | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,651.53.3 | 1,626.0 | 25.5 | 1,626.0 | 25.5 |
| 21 | −1 | 0.400 | −1 | 1.00 | −1 | 7.00 | 1,081.82.9 | 1,127.2 | –45.4 | 1,080.3 | 1.5 |
| 22 | 1 | 0.700 | −1 | 1.00 | −1 | 7.00 | 1,063.42.8 | 1,035.9 | 27.5 | 1,069.0 | –5.6 |
| 23 | −1 | 0.400 | 1 | 2.00 | −1 | 7.00 | 983.11.3 | 947.2 | 35.9 | 980.2 | 2.9 |
| 24 | 1 | 0.700 | 1 | 2.00 | −1 | 7.00 | 647.53.1 | 658.0 | –10.5 | 647.9 | –0.4 |
| 25* | −1 | 0.400 | −1 | 1.00 | 1 | 14.00 | 1,181.81.6 | 1,136.2 | 45.6 | 1,149.1 | 32.7 |
| 26* | 1 | 0.700 | −1 | 1.00 | 1 | 14.00 | 1,103.11.5 | 1,137.7 | –34.6 | 1,091.6 | 11.5 |
| 27 | −1 | 0.400 | 1 | 2.00 | 1 | 14.00 | 1,190.24.4 | 1,225.5 | –35.3 | 1,189.4 | 0.8 |
| 28 | 1 | 0.700 | 1 | 2.00 | 1 | 14.00 | 1,058.73.8 | 1,029.3 | 29.4 | 1,055.6 | 3.1 |
| 29* | −1.682 | 0.298 | 0 | 1.50 | 0 | 10.50 | 1,039.12.0 | 1,025.1 | 14.0 | 1,039.9 | –0.8 |
| 30 | 1.682 | 0.802 | 0 | 1.50 | 0 | 10.50 | 761.02.6 | 783.3 | –22.3 | 762.1 | –1.1 |
| 31 | 0 | 0.550 | −1.682 | 0.66 | 0 | 10.50 | 1,308.51.8 | 1,280.7 | 27.8 | 1,298.1 | 10.4 |
| 32 | 0 | 0.550 | 1.682 | 2.34 | 0 | 10.50 | 1,039.21.1 | 1,038.1 | 1.1 | 1,030.5 | 8.7 |
| 33 | 0 | 0.550 | 0 | 1.50 | −1.682 | 4.61 | 997.61.2 | 989.0 | 8.6 | 994.0 | 3.6 |
| 34 | 0 | 0.550 | 0 | 1.50 | 1.682 | 16.39 | 1,338.92.4 | 1,308.7 | 30.2 | 1,337.8 | 1.1 |
| 35 | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,711.61.6 | 1,626.0 | 85.6 | 1,626.0 | 85.6 |
| 36* | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,563.23.7 | 1,626.0 | –62.8 | 1,626.0 | –62.8 |
| 37 | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,549.45.2 | 1,626.0 | –76.6 | 1,626.0 | –76.6 |
| 38 | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,640.63.2 | 1,626.0 | 14.6 | 1,626.0 | 14.6 |
| 39* | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,699.34.1 | 1,626.0 | 73.3 | 1,626.0 | 73.3 |
| 40* | 0 | 0.550 | 0 | 1.50 | 0 | 10.50 | 1,588.74.5 | 1,626.0 | –37.3 | 1,626.0 | –37.3 |
*Runs that were selected randomly to serve for validating the model of ANN; the other 22 runs served for model training.