FIG 1.

UQ₈ is the major quinone used by F. novicida. (A) HPLC-ECD analysis of lipid extracts from 1 mg of E. coli MG1655 (E.c.) and F. novicida (F.n.) cells grown aerobically in Chamberlain medium with 0.4% (wt/vol) glucose or succinate as the sole carbon source. The chromatograms are representative of results from three independent experiments. The peaks corresponding to UQ₈, DMK₈, and the UQ₁₀ standard are indicated. (B) Mass spectrum of the quinone eluting at 8.30 min from extracts of F. novicida grown in Chamberlain medium. H⁺, NH₄⁺, and Na⁺ adducts of UQ₈ are indicated.