FIG 5.
UQ8 is essential for the growth of F. novicida in respiratory medium. (A and B) F. novicida (F.n.) and a transposon mutant of ubiCFn (Tn-ubiCFn) were grown aerobically in Chamberlain medium with 0.4% (wt/vol) succinate (A) or glucose (B) as the sole carbon source. Growth (average from sextuplicate growth curves) was monitored as the change in the absorbance at 600 nm in a Tecan plate reader. (C) Cellular UQ8 contents were quantified for F. novicida and the Tn-ubiCFn mutant according to methods described in Materials and Methods. 4HB was added to rescue the growth and UQ8 biosynthesis of the Tn-ubiCFn mutant. Quantifications are expressed as picomoles per milligram of cells (n = 3). ****, P < 0.0001 (by unpaired Student’s t test).