TABLE 2.
Doubling times for isogenic E. coli strains bearing suppressors of the Δhda growth defect
| Straina | Relevant genotypec |
Doubling time (min)b | |||
|---|---|---|---|---|---|
| dnaN | diaA | pRM100 (Pol I) | hda | ||
| VB001 | + | + | − | + | 41.1 ± 2.6 |
| VB002 | + | Δ | − | + | 41.1 ± 2.6 |
| VB003 | + | Δ | − | Δ | 41.2 ± 1.6 |
| VB001 (pRM100) | + | + | + | + | 42.5 ± 3.3 |
| VB004 | + | + | + | Δ | 42.9 ± 2.6 |
| VB019 | E202K | + | − | + | 43.1 ± 1.8 |
| VB020 | E202K | Δ | − | + | 44.3 ± 2.8* |
| VB021 | E202K | Δ | − | Δ | 43.3 ± 3.3 |
| VB019 (pRM100) | E202K | + | + | + | 44.7 ± 3.9 |
| VB022 | E202K | + | + | Δ | 47.0 ± 2.7** |
a
Strains are described in Table 5.
b
Doubling time represents the average of 8 replicates ± SD. Cultures were grown in a sterile microtiter plate, so doubling times are slightly longer than they would be than if grown in tubes with more efficient aeration. *, P ≤ 0.05; **, P ≤ 0.001 (Student's t test) relative to the wild-type VB001 control.
c
Symbols: +, wild-type gene or pRM100 is present; Δ, gene has been deleted; −, pRM100 is absent.