FIG 1.

Growth of the WT, Δicl1, Δicl2, and Δicl1 Δicl2 strains of M. smegmatis on various carbon sources. The Δicl1, Δicl2, and Δicl1 Δicl2 mutant strains, as well as the WT strain as a control, were grown aerobically at 37°C in 7H9 medium supplemented with 10 mM glucose, 10 mM acetate, or 2.5 mM octanoate as the sole carbon source. For complementation of the Δicl1 mutant, pMV306icl1 (a pMV306-derived plasmid carrying the intact icl1 gene and its own promoter) was introduced into the Δicl1mutant. The Δicl1 strain harboring pMV306icl1, as well as the WT and Δicl1 strains with the empty vector pMV306, was grown aerobically in 7H9-acetate at 37°C. All values provided were determined from three biological replicates. The error bars indicate the standard deviations.