FIG 7.

Effects of mutations in the RamB-binding sites (RamBS1 and RamBS2) on icl1 expression. The icl1 promoter activities were determined by using the pNCIIicl1-derived translational fusion plasmids (pNCIIicl1BM1 and pNCIIicl1BM2) with mutations in RamBS1 and RamBS2, respectively. As a control, pNCIIicl1 was included in the experiment. The WT strain of M. smegmatis harboring the translational fusion plasmids was grown aerobically to an OD₆₀₀ of 0.45 to 0.5 in 7H9 medium supplemented with 10 mM glucose or 10 mM acetate as the sole carbon source. Cell-free crude extracts were used to measure β-galactosidase activity. All values provided were determined from three biological replicates. The error bars indicate the standard deviations. *, P < 0.01.