FIG 9.

Effects of the carbon source on intracellular levels of cAMP in M. smegmatis. The WT strain of M. smegmatis was grown aerobically to an OD₆₀₀ of 0.45 to 0.5 in 7H9 medium supplemented with 10 mM glucose, 10 mM acetate, or 2.5 mM octanoate as the sole carbon source. cAMP quantification was performed as described in Materials and Methods. All values provided are the average of the results from three biological replicates. The error bars indicate the standard deviations. *, P < 0.05; **, P < 0.01.