| Antibodies |
| Anti-mouse CD16/32, purified, Clone 93 |
Biolegend |
Cat#101330;RRID:AB_2783037 |
| Anti-mouse/human B220, BV711, Clone RA3-6B2 |
Biolegend |
Cat#103225;RRID: AB_492874 |
| Anti-mouse IgD, BV510, Clone 11-26c.2a |
Biolegend |
Cat#405723;RRID:AB_2562742 |
| Anti-mouse CD138, BV421, Clone 281–2 |
Biolegend |
Cat#142508;RRID:AB_11204257 |
| Anti-mouse CD38, PE-Cy7, Clone 90 |
Biolegend |
Cat#102718;RRID: AB_2072892 |
| Anti-mouse/human GL7, APC, Clone GL7 |
Biolegend |
Cat#144606;RRID:AB_2562184 |
| Anti-mouse/human GL7, PerCPCy5.5, Clone GL7 |
Biolegend |
Cat#144610;RRID: AB_2562978 |
| Anti-mouse/human GL7, AF488, Clone GL7 |
Biolegend |
Cat#144612;RRID: AB_2563284 |
| Anti-mouse IgG1, FITC, Clone RMG1-1 |
Biolegend |
Cat#406606;RRID: AB_493292 |
| Anti-mouse IgG2b, FITC, Clone RMG2b-1 |
Biolegend |
Cat#406706;RRID: AB_493296 |
| Anti-mouse CD98, AF647, Clone RL388 |
Biolegend |
Cat#128210;RRID: AB_2254922 |
| Anti-mouse CD90.1, APC, Clone OX-7 |
Biolegend |
Cat#202526;RRID: AB_1595470 |
| Anti-mouse CD184(CXCR4), PE, Clone 2B11 |
BD Biosciences |
Cat#551966;RRID: AB_394305 |
| Anti-mouse CD86, BV605, Clone GL-1 |
Biolegend |
Cat#105037;RRID:AB_11204429 |
| Anti-mouse IgA, FITC, polyclonal |
Southern Biotech |
Cat#1040-02;RRID:AB_2794370 |
| Anti-mouse IgA, purified, Clone C10-3 |
BD Biosciences |
Cat#556969;RRID:AB_396541 |
| Mouse IgA, unlabeled, Clone S107 |
Southern Biotech |
Cat#0106-01;RRID:AB_2714214 |
| Anti-mouse IgA-HRP, polyclonal |
Southern Biotech |
Cat#1040-05;RRID:AB_2714213 |
| Anti-mouse IgD, purified, polyclonal |
Nordic Immunology |
Cat#GAM/IgD(Fc)/7S |
| Anti-mouse/human GL7, biotin, Clone GL7 |
Biolegend |
Cat#144616;RRID: AB_2721505 |
| Anti-mouse CD21/CD35, AF647, Clone 7E9 |
Biolegend |
Cat#123424;RRID: AB_2629577 |
| Anti-mouse CD35, biotin, Clone 8C12 |
BD Biosciences |
Cat#553816;RRID:AB_395068 |
| Anti-goat Donkey-HRP, polyclonal |
Jackson Immunoresearch |
Cat#705-035-147;RRID: AB_2313587 |
| Anti-biotin-AP, 3D6.6 |
Jackson Immunoresearch |
Cat#200-052-211;RRID: AB_2339015 |
| Anti-goat Cy3, polyclonal |
Jackson Immunoresearch |
Cat#705-166-147;RRID: AB_2340413 |
| Anti-rabbit AF488, polyclonal |
Jackson Immunoresearch |
Cat#711-546-152; RRID:AB_2340619 |
| Anti-mouse CXCR5, BV421, Clone L138D7 |
Biolegend |
Cat#145512;RRID: AB_2562127 |
| Anti-mouse PD-1, PE-Cy7, Clone 29F.1A12 |
Biolegend |
Cat#135215;RRID:AB_10696422 |
| Anti-mouse CD4, FITC, Clone RM4-4 |
Biolegend |
Cat#116004;RRID: AB_313688 |
| Anti-mouse TCRbeta, BV605, Clone H57-597 |
Biolegend |
Cat#109241;RRID: AB_2629563 |
| Anti-mouse/human SREBP2 (a.a. 843–857), purified, polyclonal |
Invitrogen |
Cat#PA1-338;RRID:AB_2194237 |
| Anti-SREPB2, purified, Clone 22D5 |
Millipore Sigma |
Cat#MABS-1988 |
| Anti-mouse/human SREBP2 (a.a. 1–220), purified, polyclonal |
Invitrogen |
Cat#PA5-88943;RRID: AB_2805240 |
| Anti-mouse/human IRF4, EF660, Clone 3E4 |
Invitrogen |
Cat#50-9858-82;RRID: AB_2574393 |
| Bacterial and virus strains |
|
S. enterica strain SL1344 |
Gift from M. Bogunovic |
N/A |
|
S. enterica strain SL1344 aroA
|
Gift from M. Bogunovic |
N/A |
| Chemicals, peptides, and recombinant proteins |
| Anti-IgM |
Jackson ImmunoResearch Laboratories Inc. |
Cat# 115-006-075 |
| Anti-CD40 |
Bio X Cell |
Cat# BE0016-2 |
| DAPI |
Thermo Fisher |
Cat#1306 |
| ER-Tracker |
Invitrogen |
Cat# E12353
|
| FTY-720 |
Selleck Chemicals |
Cat# S5002 |
| Cholera toxin |
List Biological Laboratories |
Cat#100B |
| Diphtheria toxin |
Sigma |
Cat#D0564 |
| 25-hydroxycholesterol |
Avanti Polar Lipids |
Cat# 700019P |
| 7a,25-dihydroxycholesterol |
Avanti Polar Lipids |
Cat# 700080P |
| Ampicillin |
Corning |
Cat# 61-238-RH |
| Metronidazole |
MP Biomedicals |
Cat#155710 |
| Neomycin |
SIGMA |
Cat#N1876 |
| Vancomycin |
Fisher Bioreagents |
Cat#BP2958-1 |
| Ibrutinib |
Selleckchem |
Cat# S2680 |
| R406 |
Invivogen |
Cat# inhr406 |
| API1 |
Sigma |
Cat# SML1342 |
| Rapamycin |
LC Laboratories |
Cat#R-5000 |
| Mevastatin |
Cayman Chemical |
Cat# 73573-88-3 |
| LTbR-hIgG-Fc |
This paper |
N/A |
| hIgG-Fc |
This paper |
N/A |
| 5-Fluorouracil |
Sigma |
Cat# F6627 |
| Collagenase IV |
Worthington |
Cat# LS004189
|
| DNase I |
Sigma |
Cat# DN25 |
| Percoll |
Ge Healthcare |
Cat# 17089101 |
| Trizol |
Invitrogen |
Cat#10296028 |
| Critical commercial assays |
| BD Cytofix/Cytoperm solution |
BD Biosciences |
Cat# 554714 |
| SYBR green master mix |
Biorad |
Cat# 1725270 |
| Script First-Strand kit |
Invitrogen |
Cat# 11904-018 |
| Elisa/Elispot diluent solution |
Invitrogen |
Cat#004202 |
| Fluoromount-G |
SouthernBiotech |
Cat#0100-01 |
| EasySep mouse B cell isolation kit |
Stemcell |
Cat#19854A |
| Experimental models: Cell lines |
| Human Embryonic Kidney Cells (HEK293T) |
ATCC |
Cat# CRL-3216 |
| M12-EBI2-GFP |
Gift from J. Cyster |
Kelly et al. 2011 |
| WEHI-231 |
ATCC |
Cat# CRL-1702 |
| Platinum-E (Plat-E) Retroviral Packaging Cell Line |
Gift from S. Schwab |
N/A |
| NB-21 |
Gift from G. Kelsoe |
Kuraoka M.et al.2016 |
| Experimental models: Organisms/strains |
| Mouse: C57BL/6J |
The Jackson Laboratory |
000664 |
| Mouse: B6.SJL-Ptprca
Pepcb/BoyJ |
The Jackson Laboratory |
002014 |
| Mouse: Rosa26-flox-stop-flox-DTR
|
The Jackson Laboratory |
007900 |
| Mouse: Srebf2 flox/flox
|
The Jackson Laboratory |
031792 |
| Mouse: Ch25h−/−
|
The Jackson Laboratory |
016263 |
| Mouse: Gpr183−/−
|
Pereira et al., 2009 |
N/A |
| Mouse: Aicda-cre
|
The Jackson Laboratory |
007770 |
| Mouse: Cd21-Cre
|
The Jackson Laboratory |
006368 |
| Mouse: Rosa26-flox-stop-flox-tdTomato
|
The Jackson Laboratory |
007914 |
| Oligonucleotides |
| See table S1 for list of quantitative RT-PCR primers |
N/A |
N/A |
| Recombinant DNA |
| MSCV-IRES-Thy1.1 |
Addgene |
Plasmid ID: 17442 |
| MSCV-Flox-STOP-Flox-IRES-Thy1.1 |
Gift from J. Cyster |
Green et al. 2011 |
| MSCV-IRES-H2B-RFP |
Gift from J. Cyster |
N/A |
| Software and algorithms |
| FlowJo V 10.6 software |
Tree Star |
https://www.flowjo.com/solutions/flowjo/downloads
|
| FACSDiva V 7.0 software |
BD |
https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software
|
| ZEN 3.1 |
Carl Zeiss Microscopy |
https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html
|
| Adobe Illustrator 2019 |
Adobe Systems |
N/A |
| ImageJ |
NIH |
https://imagej.nih.gov/ij/
|
| Prism 9 |
GraphPad Software |
http://www.graphpad.com/scientific-software/prism/
|
| Other |
| standard chow diet |
LabDiet |
Cat#5P76 |
| 0.15% high cholesterol diet |
Envigo TD |
Cat#180381 |
