FIG. 8.

Yng2-associated HAT activity is deficient in esa1(Ts) cells. Extracts from cells containing a control plasmid pAD4.H (vector), or expressing HA-Yng2 (Yng2) or GFP-HA-Gcn5 (Gcn5) were immunoprecipitated with anti-HA. Immunoprecipitates were split, and half of each sample was examined by Western blot analysis using anti-HA antibody (top), and half was assayed for HAT activity (bottom). Strains used include FY105 (WT), gcn5Δ, LPY3498 grown at 30°C (WT 30) or 37°C (WT 37), LPY3291 grown at 30°C (esa1-1 30) or 37°C (esa1-1 37), and LPY3500 grown at 30°C (esa1-2 30) or 37°C (esa1-2 37).