REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse HRP	DAKO	K4001; RRID:AB_2827819
Anti-rabbit HRP	DAKO	K4003; RRID:AB_2630375
Anti-rat HRP	Vectorlab	MP-7404-50; RRID:AB_2336531
Armenian hamster anti-CD11c (clone N418)	BioLegend	117328, 117308, 117343, 117309, 117312; RRID:AB_2129641, RRID:AB_313777, RRID:AB_2563099, RRID:AB_313778, RRID:AB_389328
Armenian hamster anti-CD11c (clone N418)	eBioscience	25-0114-82; RRID:AB_10372340
Armenian hamster anti-CD3 (clone 145-2C11)	ThermoFisher, BioLegend	11-0031-82, 100328, RRID:AB_464882, RRID:AB_893318
Armenian Hamster anti-CD3 (clone 145-2C11)	BioLegend	100314; RRID:AB_312679
Armenian hamster anti-CD49a (clone Ha31/8)	BD	564667; RRID:AB_2722549
Mouse anti-APP A4 a.a. 66-81 (clone 22C11)	Sigma (formerly Millipore)	MAB348; RRID:AB_94882
Mouse anti-CD64 (clone X54-5/7.1)	BioLegend	139308, 139309; RRID:AB_2561963, RRID:AB_2562694
Mouse anti-CX3CR1 (clone SA011F11)	BioLegend	149013, 123135; RRID:AB_2565697, RRID:AB_2562622
Mouse anti-NK1.1 (clone PK136)	BioLegend	108727; RRID:AB_2132706
Mouse anti-XCR1 (clone ZET)	BioLegend	148207; RRID:AB_2564363
Rabbit anti-CD3e (polyclonal)	DAKO	A0452; RRID:AB_2335677
Rat anti-B220 (clone RA3-6B2)	BioLegend	103207; RRID:AB_312992
Rat anti-CCR2 (MC-21)	Provided by Matthias Mack	N/A
Rat anti-CD103 (clone M290)	BD	564322; RRID:AB_2738744
Rat anti-CD107b (Mac3) (clone M3/84)	BioLegend	108502; RRID:AB_313383
Rat anti-CD115 (clone AFS98)	BioLegend	135505, 135515, 135523; RRID:AB_1937254, RRID:AB_2562679, RRID:AB_2566459
Rat anti-CD117 (clone ACK2, 2B8)	BioLegend	135111, 105827; RRID:AB_2131136, RRID:AB_10898120
Rat anti-CD11b (clone M1/70)	BioLegend	101242, 101228, 101222, 101263; RRID:AB_2563310, RRID:AB_893232, RRID:AB_493705, RRID:AB_2629529
Rat anti-CD11b (clone M1/70)	BD	553310; RRID:AB_394774
Rat anti-CD127 (clone A7R34)	BioLegend	135009; RRID:AB_1937252
Rat anti-CD135 (clone A2F10)	BioLegend	135310; RRID:AB_2107050
Rat anti-CD16/32 TruStain FcX (clone 93)	BioLegend	101320; RRID:AB_1574975
Rat anti-CD19 (clone 6D5)	BioLegend	115534; RRID:AB_2072925
Rat anti-CD4 (clone GK1.5)	BioLegend	100422; RRID:AB_312707
Rat anti-CD45 (clone 30-F11)	BioLegend	103114, 103108; RRID:AB_312979, RRID:AB_312973
Rat anti-CD8 (clone 53-6.7)	BD	563786; RRID:AB_2732919
Rat anti-F4/80 (clone BM8)	BioLegend	123114; RRID:AB_893478
Rat anti-F4/80 (clone BM8)	BioLegend	107622, RRID:AB_493727
Rat anti-GM-CSF (clone MP1-22E9)	ThermoFisher	11733181; RRID:AB_465420
Rat anti-IFNg (clone XMG1.2)	BioLegend	508510, 505830; N/A, RRID:AB_2563105
Rat anti-IFNg (XMG1.2)	Bio X Cell	BE0055; RRID:AB_1107694
Rat anti-IL-17 (clone TC11-18H10.1)	BioLegend	506904; RRID:AB_315464
Rat anti-Ly6C (clone HK1.4)	BioLegend	128036, 128037; RRID:AB_2562353; RRID:AB_2562630
Rat anti-Ly6G (clone 1A8)	BioLegend	127622, 126615, 565707; RRID:AB_10643269, RRID:AB_2562776, N/A
Rat anti-MHCII (clone M5/114.15.2)	BioLegend	107620, 107606, 107631, 107622; RRID:AB_493527, RRID:AB_313321, RRID:AB_10900075, RRID:AB_493727
Rat anti-mouse CD49d (clone R1-2)	BioLegend	149027; RRID:AB_2565937
Rat anti-NOS2 (clone CXNFT)	ThermoFisher	53-5920-82; RRID:AB_2574423
Rat anti-PDCA-1 (clone 927)	BioLegend	127012; RRID:AB_1953287
Rat anti-Sca1 (clone D7)	BioLegend	108116; RRID:AB_493269
Rat anti-Siglec-F (clone E50-2440)	BD	552126; RRID:AB_394341
Rat anti-Ter119 (clone Ter119)	BioLegend	116228; RRID:AB_893636
Rat IgG2b isotype	Bio X Cell	BE0090; RRID:AB_1107780
Sheep anti-Arg1	R&D	IC5868P
Syrian Hamster anti-CD28 (clone 37.51)	BioLegend	102112; RRID:AB_312877
Chemicals, peptides, recombinant proteins
2-Mercaptoethanol	Biorad	1610710
Acetone	Sigma	179124
Brefeldin A	BioLegend	420601
CL316,243	Sigma	C5976
Collagenase A	Roche	11088882001
Collagenase D	Sigma	11088866001
DAB	DAKO	K5001
DAPI	ThermoFisher	D1306
Dibutyl phthalate,99%	Sigma	524980
DMEM	ThermoFisher	41965-039
DNase	Roche	10104159001
FA Folic Acid solution	Sigma	F8758
Fluorescein isothiocyanate isomer I	Sigma	F7250
Hemalun	Merck	1092490.500
Heparin	Sigma	H3393
hTGF-beta	Peprotech	100-21C
IL-12	Peprotech	210-12
IL-18	MBL (Biozol)	B004-5
IL-2	ebioscience	34-8021-82
IL-23	Miltenyi	130-096-676
IL-6	Peprotech	216-16
IL-7	Peprotech	217-17
Ionomycin	Sigma	IO634
L-Arginine	Sigma	11009
L-Asparagine	Sigma	A0884
L-Glutamin	Bioconcept	5-10K00H
Lithium Carbonate	Merck	5671.0250
Luxol Fast Blue	BDH laboratories	340443P
Monensin	BioLegend	420701
NEAA 100x	GIBCO	11140-035
Penicilin-Streptomycin solution	AMIMED	4-01F-00H
Percoll	GE Healthcare	17-0891-01
Periodoc Acid	Merck	1005240100
PMA	Sigma	P1585
Schiff’s reagent	Merck	1.09033.0500
Sodium Pyruvate	Sigma	S8636
Thioglycollate medium	Sigma	T9032
VIT Vitamin mix solution	Sigma	M6985
Critical commercial assays
AccuCheck Counting Beads	Molecular Probes	PCB100
BD FACS Lysing Solution	BD	349202
CD4(l3t4) microbeads mouse	Miltenyi	130-117-043
cDNA Reverse Transcription Kit	Applied Biosystems	4368814
Fixation buffer	BioLegend	420801
FoxP3/Transcription Factor Staining Buffer Set	eBioscience	00-5523-00
heat inactivated mycobacterium tuberculosis H3/RA	BD Difco	231141
Incomplete Freund`s adjuvant	BD Difco	263910
Intracellular Staining Perm Wash Buffer	BioLegend	421002
MOG peptide 35-55	Peptides&elephants	N/A
Nextera kit	Illumina	N/A
Pertussis toxin	Sigma/ Calbiochem	P7208/ 516560
RBC Lysis buffer	BioLegend	420301
RNAlater stabilization solution	ThermoFisher	AM7020
RNeasy plus Micro Kit	QIAGEN	74034
RNeasy plus Mini Kit	QIAGEN	74134
PowerUp SYBR Green Master Mix	Applied Biosystems	A25741
SMART-Seq v4 ultra-low input kit	Clontech	N/A
TRIzol reagent	ThermoFisher	15596026
TruSeq stranded mRNA kit	Illumina	N/A
Versacomp beads	Beckman Coulter	B22804
Zombie NIR Fixable Viability Kit	BioLegend	423105
Deposited data
RNA sequencing data	This paper	GEO: GSE172021
RNA sequencing data	This paper	GEO: GSE183321
Experimental models: Cell lines
B16-GMCSF cells	Dranoff et al., 1993	N/A
Experimental models: Organisms/strains
Mouse: 2D2	The Jackson Laboratory	006912
Mouse: C57BL/6	Charles River, Janvier	N/A
Mouse: Ccr2-KO	The Jackson Laboratory	004999
Mouse: Ucp1-KO	The Jackson Laboratory	003124
Oligonucleotides
M. musculus Ucp1	5′-GGCCTCTACGACTCAGTCCA-3′	5′-TAAGCCGGCTGAGATCTTGT-3′
M. musculus Cidea	5′-TGCTCTTCTGTATCGCCCAGT-3′	5′-GCCGTGTTAAGGAATCTGCTG-3′
M. musculus Ppargc1a	5′-AAGTGTGGAACTCTCTGGAACTG-3′	5′-GGGTTATCTTGGTTGGCTTTATG-3′
M. musculus Ppara	5′-AGAGCCCCATCTGTCCTCTC-3′	5′-ACTGGTAGTCTGCAAAACCAAA-3′
M. musculus Pparg	5′-AGGCGAGGGCGATCTTGACAG-3′	5′-AATTCGGATGGCCACCTCTTTG-3′
M. musculus Prdm16	5′-CAGCACGGTGAAGCCATT-3′	5′-GCGTGCATCCGCTTGTG-3′
M. musculus 36B4	5′- GTACCCATTGATGATGGAGTGT-3′	5′-TGAAGTGCTCGACATCACAG-3′
M. musculus B2m	5′-TTGTCTCACTGACCGGCCT-3′	5′-TATGTTCGGCTTCCCATTCTCC-3′
Software and Algorithms
FastQC v.0.11.5	Babraham bioinformatics	https://www.bioinformatics.babraham.ac.uk/projects/fastqc/
FLIR Tools+ software	FLIR technologies	https://www.flir.com/products/flir-tools/
FlowJo 10.2	TreeStar	https://www.flowjo.com/solutions/flowjo/downloads/previous-versions
GraphPad 8	Prism	https://www.graphpad.com/scientific-software/prism/
HTSeq v0.9.1	HTSeq	https://htseq.readthedocs.io/en/master/
MetaCore	Clarivate Analytics	https://clarivate.com/cortellis/wp-content/uploads/sites/4/2020/02/MetaCore.pdf
Pannoramic Viewer software	3DHistech	https://www.3dhistech.com/research/software-downloads/
Picard tools v.1.141	N/A	https://broadinstitute.github.io/picard/
QuPath v0.2.2	QuPath	https://qupath.github.io/QuPath-v0.2.0.html
R language and environment for statistical computing and graphics	N/A	https://www.r-project.org
R/Bioconductor package EdgeR v. 3.4.2	(McCarthy et al., 2012)	https://bioconductor.org/packages/release/bioc/html/ReactomePA.html
R/Bioconductor package topGO	N/A	https://bioconductor.org/packages/release/bioc/vignettes/topGO/inst/doc/topGO.pdf
ReactomePA R-package	(Yu and He, 2016)	https://bioconductor.org/packages/release/bioc/html/ReactomePA.html
Rgraphviz	(Hansen et al., 2021)	https://www.bioconductor.org/packages/release/bioc/html/Rgraphviz.html
STAR aligner v.2.6.0c	(Dobin et al., 2013)	N/A
Seahorse Wave Desktop Software	Agilent	N/A
VivoQuant v.2020-build9	inviCRO	http://www.vivoquant.com/
Other
Attune NxT	ThermoFisher	N/A
AutoMACS Pro	Miltenyi	N/A
AxioScan Z1	Zeiss	N/A
Bioanalyzer Agilent 2100	Agilent	https://www.agilent.com/en/product/automated-electrophoresis/bioanalyzer-systems/bioanalyzer-instrument/2100-bioanalyzer-instrument-228250
BD FACSAria II	BD Biosciences	N/A
BD FACSymphony	BD Biosciences	N/A
BD LSRFortessa	BD Biosciences	N/A
TProfessional Basic Thermocycler	Biometra	N/A
Bio-Rad S3	Bio-Rad	N/A
FLIR E60 thermal camera	FLIR systems	N/A
HiSeq 4000	Illumina	N/A
Light Cycler 480-II	Roche	N/A
MoFlo Astrios	Beckman Coulter	N/A
NanoDrop	ThermoFisher	N/A
Pannoramic 250 FLASH II	3Dhistech	N/A
Seahorse XF24 Analyzer	Agilent	N/A
Tissue Lyser II	QIAGEN	N/A
Triumph PET/SPECT/CT	TriFoil Imaging	N/A