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. 2021 Oct 26;17(10):e1009693. doi: 10.1371/journal.ppat.1009693

Fig 2. . S. epidermidis and S. xylosus colonization before L. major infection increase the inflammatory responses and IL-17A+ILCs.

Fig 2

(A) Schematic representation of S. epidermidis (S. epi) and L. major treatment protocol in C57BL/6 mice. (B) Recovered total colony forming units (CFUs) in the ear of different treatment groups at week two. (C) Recovered pink (S. epi-2W) colony forming units (CFUs) in the ear of different treatment groups at week two. (D) Ear thickness measurement and pathology score in mice associated with S. epi or unassociated prior to L. major infection at week one and two. (E) Percent of IL-17Α+ILCs in the skin of different treatment groups at week two. (F) Number of IL-17Α+ILCs in the skin of different treatment groups at week two. (G) Ki67 staining on IL-17A+ ILCs in S. epi colonized and L. major infected mice at week two. Total ILCs were used as isotype. (H) Percent of RORγt+ILCs in the skin of different treatment groups at week two. (I) IL-17A production from RORγt+ILCs and RORγt-ILCs in S. epi colonized and L. major infected mice at week two. (J) Number of RORγt+ILCs in the skin of different treatment groups at week two. (K) Number of RORγt+IL-17A+ILCs in the skin of different treatment groups at week two. (L) Ear thickness measurement and pathology score in mice associated with S. xylosus (S. xylo) or unassociated prior to L. major infection at week one and two. (M, N) Percent and number of IL-17Α+ILCs in the skin of different treatment groups at week two. The number within the flow plots show percent of IL-17A+ cells (E,M) or RORγt+ cells (H) with SEM or IL-17A+ cells with in the gated box (I). Data are from three experiments with a total of 10 to 16 mice in each group (E,F,M,N) except control groups in N which is from one experiment with three mice or from one experiment representative of two with five mice in each group (B,C,D,L) or from one experiment with three to five mice in each group (G,H,J,K). Error bars shows SEM (B,H,I.J,N) or SD (D,L). Two-tailed unpaired Student’s t-test with Welch’s correction or one-way ANOVA with Tukey’s multiple comparison analysis (B,F,N). ns, not significant; *p<0.05, **p<0.01, ***p<0.001.