Skip to main content
. 2021 Oct 26;17(10):e1009865. doi: 10.1371/journal.pgen.1009865

Fig 4. Allelic differences in transcriptional activity for a context-dependent regulatory variant in a context-dependent element at the SCD locus.

Fig 4

(A) Peak19405 (red) is more accessible in D4 and D14 adipocytes than D0 preadipocytes, overlaps an adipose tissue consensus peak (dark purple), and overlaps variant rs603424, which is associated with blood plasma levels of palmitoleic acid and adipose SCD expression. SCD is also more highly expressed at D4 and D14 compared to D0. Additional tracks show adipose tissue ATAC-seq from ENCODE (light purple) and adipose nuclei histone mark ChIP-seq from the Roadmap Epigenomics project (blue and green). (B) A 592-bp genomic region surrounding peak19405 containing the rs603424-G allele shows increased transcriptional activity compared to the rs603424-A allele in the forward and reverse orientations only in adipocytes (tested at day 12), the context in which chromatin was more accessible compared to preadipocytes. Dots represent two independent constructs assayed from four replicates each. Luciferase activity was normalized relative to an empty vector (EV). Values in S18 Table.