. Author manuscript; available in PMC: 2022 Jul 23.

Published in final edited form as: ACS Appl Nano Mater. 2021 Jul 12;4(7):7343–7357. doi: 10.1021/acsanm.1c01320

Table 3.

Ligand Density and Binding Affinity of Man- and G7-Glycoliposomes with Con A

entry	glycoliposome composition	types of liposomes	glycolipid in glycoliposomes (μmol/mg)^a	size of glycoliposomes (nm)^b	B_max (10³ nm)^c	h ^c	K^d (μM)^c	affinity enhancement^d
1	PC/Chol/Man-DPPE (2:1:0.2)	nonfluid	66	128 ± 12	4.9	4.6	1.1	309
2	PC/Chol/G7-DPPE (2:1:0.2)	nonfluid	21	147 ± 11	6.4	7.0	0.93	892
3	DPPC/DMPG/Man-DPPE (6:1:0.6)	fluid	59	118 ± 15	8.7	5.1	2.9	117
4	DPPC/DMPG/G7-DPPE (6:1:0.6)	fluid	14	136 ± 8	7.0	2.8	0.51	1627

Determined by the anthrone–sulfuric acid assay.

Measured by DLS. Data are presented as mean ± SD.

Obtained from fitting the data in Figure 2 to eq 1.

Affinity enhancement = K_D/K_d, where K_D is 0.34 mM for Con A–Man and 0.83 mM for Con A–G7.⁶²