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. Author manuscript; available in PMC: 2022 Mar 19.
Published in final edited form as: ACS Catal. 2021 Mar 1;11(6):3319–3334. doi: 10.1021/acscatal.0c04886

Figure 1. Cysteine polysulfide as the sulfur source in EanB-catalysis.

Figure 1.

(A) EanB-MetC-ergothionase coupled reaction scheme; (B) Kinetic trace of MetC-EanB-ergothionase coupled assay. A typical 1-mL assay contained 4 nM MetC (3 equivalents of EanB activity), 1 mM cystine, 1 mM hercynine, 400 nM ergothionase (1000-fold of EanB activity) and varying concentration of EanB (2-10 μM) in 10 mM Tris-HCl, pH 8.0. The reaction was monitored at 311 nm. (C) 1H-NMR assay of EanB-MetC coupled reaction.