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. Author manuscript; available in PMC: 2022 Mar 19.
Published in final edited form as: ACS Catal. 2021 Mar 1;11(6):3319–3334. doi: 10.1021/acscatal.0c04886

Figure 2. Modification of EanB’s active site cysteine by selenium.

Figure 2.

(A) Experimental outline for detecting Cy412 perselenide modification. (B) MS/MS spectrum of tryptic peptides from EanB-MetC coupled reaction using selenocystine as substrate. The Peptide fragment ion (residue 407 to residue 417) with [M+H]2+ at 724.2805 (predicted mass-charge ratio: 724.2776) identified Cys412 with a Cys412-S-Se-acetamide modification, which suggested the formation of the Cys412 perselenide modification during the EanB-MetC coupled reaction.