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. Author manuscript; available in PMC: 2022 Mar 19.
Published in final edited form as: ACS Catal. 2021 Mar 1;11(6):3319–3334. doi: 10.1021/acscatal.0c04886

Figure 5. MS/MS analysis of EanBY353F2Tyr mutant.

Figure 5.

(A) Peptide fragment (residue 342 – residue 357) with [M+H]2+ as 959.4395 (predicted m/z: 959.4395) identified 3,5-difluro tyrosine residue at position 353, which suggests the successful incorporation of 3,5-difluro tyrosine at position 353 in EanB (B) MS/MS spectrum of tryptic peptides from EanBY353F2Tyr MetC coupled reaction using selenocystine as substrate. The Peptide fragment (residue 407 to residue 417) with [M+H]2+ as 724.2773 (predicted m/z: 724.2776) identified Cys412 as Cys412-S-Se-acetamide modification, which suggests the Cys412 perselenide modification during the EanBY353F2Tyr-MetC coupled reaction.