Table 2. Differences in gene expression between C518F FN1 heterozygous and homozygous hiCPC-derived chondrogenic pellets compared with wild type after 5 weeks of chondrogenesis.

	HETERO vs. WT*				HOMO vs. WT*
Gene	FC	Beta	SE	P	FC	Beta	SE	P
ACAN	0.57	−0.81	0.23	4.52 × 10−4	0.57	−1.10	0.24	2.62 × 10−4
ADAMTS-5	1.55	0.63	0.48	1.93 × 10−1	5.14	2.36	0.68	5.45 × 10−4
ALPL	1.41	0.50	0.56	3.79 × 10−1	2.45	1.30	0.63	4.03 × 10−2
COL10A1	1.03	0.05	0.41	9.11 × 10−1	1.86	0.90	0.56	1.09 × 10−1
FN1	0.25	−1.98	0.16	1.00 × 10−4	0.33	−1.59	0.13	1.00 × 10−4
ITGA3	1.18	−0.41	0.77	5.93 × 10−1	3.91	1.97	0.65	2.51 × 10−3
ITGA5	0.23	−2.13	0.23	1.00 × 10−4	1.01	0.02	0.24	9.34 × 10−1
ITGAV	0.66	−0.60	0.37	1.03 × 10−1	1.53	0.61	0.37	1.03 × 10−1
ITB1	0.92	−0.12	0.33	7.20 × 10−1	2.09	1.07	0.33	1.11 × 10−3
RUNX2	1.42	0.50	0.28	6.75 × 10−2	1.79	0.84	0.26	9.48 × 10−4

*Fold change (FC), Beta value, SE, and P value for expression levels (−ΔCt) of 10 genes as determined by generalized estimation equations, with gene expression levels (−ΔCt) as dependent variable and genotype as factor, when comparing FN1 heterozygous (HETERO) and FN1 homozygous (HOMO) pellets to wild-type (WT) pellets.