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. 2021 Nov 5;7(45):eabg1964. doi: 10.1126/sciadv.abg1964

Fig. 5. l-Arginine generates peroxynitrate, GAPDH nitrosylation, and protein ADP-ribosylation.

Fig. 5.

(A) Peroxynitrate levels in MDA-MB-231 and MDA-MB-231-BrM2 cells treated with vehicle, l-arginine, the SOD mimetic tempol (SOD1mim), or the combination of l-arginine and SOD1mim for 15 and 55 min. (B) Total protein nitrosylation in MDA-MB-231 cells exposed to l-arginine for 60 min. GSNO was used as a positive control. (C) SNO-GAPDH (and total GAPDH) in MDA-MB-231 and MDA-MB-468 cells exposed for 60 min to l-arginine alone or in combination with the NOS2 inhibitor 1400W. GSNO was used as a positive control. (D) DNA damage levels assessed by comet assay in MDA-MB-231 and MDA-MB-231-BrM2 cells exposed to vehicle or l-arginine for 60 min with and without Fpg. (E) Total mono– and poly–ADP-ribosylated proteins in MDA-MB-231 and MDA-MB-468 cells exposed to l-arginine alone or in combination with the NOS2 inhibitor 1400W. (F) NAD+ levels in MDA-MB-231 and MDA-MB-468 cells exposed to l-arginine alone or in combination with the PARP inhibitor olaparib. **P < 0.001 and ***P < 0.0001.