Figure 4.

Cdkn2 deletion accelerates skin tumor formation and promotes metastasis in carcinomas induced by mutant p53. (A) Tumor kinetics in mice with the following genotypes: Cdkn2a^f/f;p53^R172H/f (n=22), Cdkn2a^f/f;p53^f/f (n=27), Cdkn2a^f/f;p53^wt/wt (n=30) and Cdkn2a^wt/wt;p53^f/f (n=23). *P< 0.05 for the following comparisons: Cdkn2a^f/f;p53^R172H/f with each of the other groups; Cdkn2a^f/f;p53^f/f with Cdkn2a^f/f;p53^wt/wt. (B) Hematoxylin and eosin staining of primary tumors with the indicated genotypes. Note the spindle appearance of all tumors that lack Cdkn2a, regardless of the p53 status. The lower right panel shows a representative image for a liver metastasis that developed from a tumor induced by deletion of Cdkn2a and activation of p53^R172H. (C) Metastasis rates in mice with the indicated genotypes. P<0.05 for comparisons between Cdkn2a^f/f;p53^R172H/f and the rest of the groups.