| Cell line (Homo sapiens) |
Colorectal carcinoma |
ATCC |
HCT116 |
This cell line has been authenticated and tested negative for mycoplasma. |
| Recombinant DNA reagent |
p3xFlag-CMV-14 |
Sigma-Aldrich |
|
|
| Recombinant DNA reagent |
pmCherry-N1 |
TAKARA |
|
|
| Recombinant DNA reagent |
pSecTag2/Hygro |
Thermo Fisher Scientific |
|
|
| Recombinant DNA reagent |
pEGFP-C1 |
CLONTECH |
|
|
| Antibody |
Anti-TXNDC11(rabbit monoclonal) |
Abcam |
Cat#: ab188329 |
WB (1:500) |
| Antibody |
Anti-EDEM1 (rabbit polyclonal) |
Sigma-Aldrich |
Cat#: E8406 |
WB (1:500) |
| Antibody |
Anti-EDEM2 (rabbit polyclonal) |
Novusbio |
Cat#: NBP2-37921 |
WB (1:500) |
| Antibody |
Anti-EDEM3 (mouse monoclonal) |
Sigma-Aldrich |
Cat#: E0409 |
WB (1:500) |
| Antibody |
Anti-GRP78 antibody (rabbit polyclonal) |
Thermo Fisher Scientific |
Cat#: PA1-014A |
WB (1:1000) |
| Antibody |
Anti-HA (rabbit polyclonal) |
Recenttec |
Cat#: R4-TP1411100 |
WB (1:1000) |
| Antibody |
Anti-Flag (mouse monoclonal) |
Sigma-Aldrich |
Cat#: F3165 |
WB (1:1000) IP (2.5 μl) |
| Antibody |
Anti-Myc-direct-HRP antibody |
MBL |
Cat#: M047-7 |
WB (1:1000) |
