Figure 2.

Accumulation of stress response transcripts during the recovery phase following the application of heat stress. (a) Accumulation of transcripts during recovery following 5-min heat stress. (b) Accumulation of transcripts during recovery following 45-min heat stress. Steady-state level of transcripts was determined by quantitative real-time PCR (qPCR) analysis as described in the method part. Relative expression (Y-axis) was determined based on the threshold cycle values for target transcripts that were calculated with the CT of Ef1a as an internal control. * and **; Student’s t-test significant at p < .05 and p < .01, respectively, compared to control. Bars indicate standard error (n = 3). Shaded color on the graphs indicates heat stress treatment.