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. 2020 Jun 19;15(8):1778920. doi: 10.1080/15592324.2020.1778920

Figure 2.

Figure 2.

Identification of upstream transcription factor TaERF4a of dehydrin wzy1-2 gene. (a). Subcellular localization of TaERF4a protein in N. benthamiana leaves performed by fluorescent microscope (Leica, DM5000 B, magnificant 20×). Tobacco leaf epidermal cells agro-infiltrated with p35 S::GFP and p35 S::TaERF4a-GFP vectors were used for transient expression of fusion proteins. The fluorescence images were visualized after 48 h infiltration. (b) Transcript level of TaERF4a in different tissues collected at 14 DAP seedlings. (c) The expression of TaERF4a, wzy1-2 genes in response to PEG treatment at 14 DAP seedlings. Total RNA was extracted from leaves at the indicated times. Wheat actin gene (Accession No. AB181991) was used as an internal reference. (d) Transient luciferase activity assay of TaERF4a in N. benthamiana leaves. (e) The relative luciferase enzyme activity of TaERF4a after the PEG treatment. The experiments were performed with at least three biological repeats. Significance analysis was determined by Student’s t-test (* = P < .05, ** = P < .01).