Figure 3.
Molecular characterization of conditional D1R- and D2R-γ7 KO mice. Gαolf and DARPP immunofluorescence in sagittal brain sections of Gng7fl/fl (A,B), Gng7fl/flD1Cre+ (C,D), and Gng7fl/flD2Cre+ mice (E,F). Scale bar, 1 mm. In Gng7fl/fl, Gαolf immunoreactivity was found throughout the striatum and in MSN axonal projections to the GPi/SNr and GPe, which are the main target regions for D1R- and D2R-positive MSNs, respectively. Gng7fl/flD1Cre+ mice exhibited profound loss of Gαolf immunoreactivity in the striatum and striatal projections to the GPi and SNpr (C). In Gng7fl/flD2Cre+ mice, Gαolf immunoreactivity was reduced in the striatum and striatal projections to the Gpe (E). DARPP staining was preserved in the striatum and striatal projection areas in all genotypes (B,D,F).