Figure 4.

Molecular characterization of conditional D₁R- and D₂R-γ₇ KO mice. Representative immunoblot data and quantification of γ₇, Gα_olf Gα_s, γ₂, γ₃ and AC5 protein levels in striatal membranes of male and female Gng7^fl/flD₁Cre⁺ (A,B) and Gng7^fl/flD₂Cre⁺ mice (C,D). Each column represents a normalized ratio (fold-change) relative to total protein loading and to controls (Gng7^fl/fl). Western blot analysis confirmed that loss of γ₇ caused a reduction of striatal Gα_olf protein levels in both conditional KO mouse lines. AC5 protein was substantially preserved, although a significant reduction of AC5 protein levels was observed in Gng7^fl/flD₁Cre⁺ female mice (B). Loss of γ₇ did not cause compensatory changes of striatal Gα_s, γ₃, and γ₂ protein levels in both conditional KO mouse lines. *p < 0.05, **p < 0.01, ***p < 0.001, Gng7^fl/flD₁Cre⁺ or Gng7^fl/flD₂Cre⁺ compared with Gng7^fl/fl WT littermates (Student's t test). Data are mean ± SEM. n = 5 mice/group.