Fig. 3.

GEF inhibits age-associated thymic involution and restores the imbalance in the T cell compartment of the thymus in old mice. (A) Representative photographs of thymuses isolated from each group of mice. (B) Thymus weight. (C) Number of total thymocytes. (D) Gating strategy used for analysis of T cell subsets. Red dots represent the isotype control and black dots represent cells stained with specific antibodies (top). Representative dot plots of CD4+ and CD8+ T cells (bottom). Percentages are shown in the quadrants.(E) Bar graph showing the mean % of CD4+ and CD8+ T cells within the CD3+CD45+ leukocyte population within thymocytes. (F) Ratio of CD4/CD8 single-positive cells in the thymus. Statistical significance was decided using one-way ANOVA, followed by Tukey's post-hoc test. Values with different letters are significantly different; p < 0.05 (a > b > c > d).