Fig. 4.

GEF increases the numbers of naïve T cells and decreases those of memory T cells in old mice. (A) Gating strategy used for analysis of T cells subsets. Red dots represent isotype controls and black dots represent cells stained with specific antibodies. (B) Representative dot plots of naïve (CD44^loCD62L^hi) and central/effector memory (CD44^hi) T cells in splenocytes. Bar graph showing mean % of subpopulations of CD4+ (C) and CD8+ (D) T cells. Statistical significance was decided using one-way ANOVA, followed by Tukey's post-hoc test. Values with different letters are significantly different; p < 0.05 (a > b > c > d).