| Antibodies |
| Biotin Micro Beads |
Miltenyi Biotec |
Cat# 130-042-401 |
| CD45-Biotin |
eBiosciences |
Cat# 13-0451-81 |
| CD31-Biotin |
eBiosciences |
Cat# 13-0319-80 |
| Cleaved Caspase 3 |
Cell Signaling Technologies |
Cat# 9664 |
| Di-methyl lysine motif |
Cell Signaling Technologies |
Cat# 14117 |
| ERK1/2 |
Cell Signaling Technologies |
Cat# 4695 |
| Phospho-ERK1/2 |
Cell Signaling Technologies |
Cat# 4376 |
| FLAG |
Sigma-Aldrich |
Cat# F1804 |
| Histone H3 |
EpiCypher |
Cat# 13-0001 |
| H3K36me1 |
Abclonal |
Cat# A2364 |
| H3K36me2 |
ThermoFisher |
Cat# MA5-14867 |
| H3K36me3 |
ThermoFisher |
Cat# MA5-24687 |
| H3K27me2 |
Cell Signaling Technologies |
Cat# 9728S |
| H3K27me3 |
ThermoFisher |
Cat# MA5-11198 |
| H4K20me2 |
Cell Signaling Technologies |
Cat# 9759S |
| H4K20me3 |
Cell Signaling Technologies |
Cat# 5737S |
| H3K9me2 |
ThermoFisher |
Cat# 710815 |
| γH2AX |
ThermoFisher |
Cat# MA5-33062 |
| Ki67 |
BD Bioscience |
Cat# 550609 |
| KRas Antibody (F234) |
Santa Cruz Biotechnology |
Cat# sc-30 |
| NSD2 |
EpiCypher |
Cat# 13-0001 |
| NSD2E1099K
|
This study |
N/A |
| PTEN |
Cell Signaling Technologies |
Cat# 9188 |
| Ter119-Biotin |
eBiosciences |
Cat# 13-5921-81 |
| Tubulin |
Millipore |
Cat# 05-661 |
| Peroxidase AffiniPure Donkey Anti-Mouse IgG (H+L) |
Jackson ImmunoResearch |
Cat# 715-035-151 |
| Peroxidase AffiniPure Donkey Anti-Rabbit IgG (H+L) |
Jackson ImmunoResearch |
Cat# 711-035-152 |
| Bacterial and Virus Strains |
| DH5 |
Thermo Fisher Scientific |
Cat# K4520-1 |
| BL21 |
Thermo Fisher Scientific |
Cat# C6070-03 |
| BL21-RIL |
Agilent Technologies |
Cat# 230240 |
| Sf9 |
Thermo Fisher Scientific |
Cat# 12659017 |
| Ad5-CMV-Cre |
Baylor College of Medicine, Viral Vector Production Core |
Cat# Ad5-CMV-Cre RRID:SCR_015037 |
| Biological Samples |
| Human LUAD Tissue Array |
MD Anderson Pathology |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| RPMI 1640 Medium |
Corning |
Cat# MT10040CV |
| DMEM Medium |
Corning |
Cat# MT10017CV |
| Fetal bovine serum |
Thermo Fisher Scientific |
Cat# 10500056 |
| PBS |
Corning |
Cat# MT21031CV |
| HBSS |
Thermo Fisher Scientific |
Cat# 14025076 |
| Trypsin-EDTA 0.25% |
Corning |
Cat# MT25053CI |
| Puromycin |
Thermo Fisher Scientific |
Cat# A1113802 |
| Hygromycin B |
Corning |
Cat# 30240CR |
| G418 Sulfate |
Corning |
Cat# MT30234CI |
| Complete Protease Inhibitor Cocktail |
Sigma-Aldrich |
Cat# 4693159001 |
| Phosphatase Inhibitor Cocktail |
Thermo Fisher Scientific |
Cat# 78420 |
| TRIzol Reagent |
Invitrogen |
Cat# 15596018 |
| Bovine Serum Albumin (BSA) |
Thermo Fisher Scientific |
Cat# BP9703100 |
| Matrigel |
Corning |
Cat# 354248 |
| L-Reduced glutathione |
Sigma-Aldrich |
Cat# G4251-25G |
| S-adenosyl-methionine |
New England Biolabs |
Cat# B9003S |
| S-Adenosyl-l-[methyl-3H] methionine |
American Radiolabeled Chemicals |
Cat# ART0288 |
| TransIT-293 |
Mirus Bio |
Cat# MIR-2706 |
| NP-40 |
Sigma-Aldrich |
Cat# I8896 |
| Phenylmethylsulfonyl fluoride (PMSF) |
Sigma-Aldrich |
Cat# P7626 |
| cOmplete, EDTA-free protease inhibitor |
Sigma-Aldrich |
Cat# 5056489001 |
| Trametinib (GSK1120212) |
Selleckchem |
Cat# S2673 |
| Tamoxifen |
Sigma-Aldrich |
Cat# T5648 |
| Recombinant nucleosome |
Epicypher |
Cat# 16-0006 |
| DMSO |
Sigma-Aldrich |
Cat# D5879 |
| 16% Formaldehyde (w/v) |
Sigma-Aldrich |
Cat# F8775 |
| PVDF membrane (0.2 μm) |
BioRad |
Cat# 1620177 |
| PVDF membrane (0.45 μm) |
Millipore |
Cat# IPVH00010 |
| Glutathione Sepharose 4B |
Sigma-Aldrich |
Cat# GE17-0756-01 |
| Coomassie Plus Assay |
Thermo Fisher Scientific |
Cat# 23236 |
| Coomassie GelCode Blue |
Thermo Fisher Scientific |
Cat# 24590 |
| Polybrene |
Sigma-Aldrich |
Cat# TR-1003-G |
| Hydroxypropyl methylcellulose |
Sigma-Aldrich |
Cat# 09963 |
| Doxycycline hyclate diet |
Envigo Teklad |
Cat# TD.01306 |
| Recombinant STAT3 protein |
Active Motif |
Cat# NC1851469 |
| Critical Commercial Assays |
| RNeasy Mini Kit |
Qiagen |
Cat# 74106 |
| ZymoPURE Plasmid Miniprep Kit |
Zymo |
Cat# D4211 |
| ZymoPURE II Plasmid Maxiprep Kit |
Zymo |
Cat# D4203 |
| DNA PCR Purification Kit |
Qiagen |
Cat# 28106 |
| DAB Substrate Kit |
Abcam |
Cat# ab64238 |
| Vectastain ABC kit |
Vector Laboratories |
Cat# PK-6100 |
| BCA Protein Assay Kit |
Pierce |
Cat# 23227 |
| ECL Substrate |
Amersham |
Cat# RPN2106 |
| PCR Mycoplasma Test Kit I/C |
PromoKine |
Cat# PK-CA91-1096 |
| Site-directed mutagenesis kit |
Agilent |
Cat# 200523 |
| Superscript First-strand Synthesis kit |
Invitrogen |
Cat# 18091050 |
| Bac-to-Bac Baculovirus Expression System |
Thermo Fisher Scientific |
Cat# 10359016 |
| PowerUP SYBR Green |
Thermo Fisher Scientific |
Cat# A25742 |
| Qiagen MinElute PCR purification kit |
Qiagen |
Cat# 28004 |
| NEBNext Ultra II DNA Library Prep Kit |
Illumina |
Cat# NEB E7645L |
| Deposited Data |
| RNA-seq and CUT&RUN |
This study |
GEO accession # GSE171218
|
| Original Blot Images Data |
This study |
Mendeley Data DOI: DOI: 10.17632/4ns5ffk8fg.1
|
| Experimental Models: Cell Lines |
| Human: A549 |
ATCC |
Cat# CRL- CCL-185 |
| Human: U2OS |
ATCC |
Cat# CRL- HTB-96 |
| Human: HT1080 |
ATCC |
Cat# CCL-121 |
| Human: 293T |
ATCC |
Cat# CRL-3216 |
| Mouse: Kras (KrasG12D) |
This study |
N/A |
| Mouse: KN (Kras;NSD2E1099K) |
This study |
N/A |
| Experimental Models: Organisms/Strains |
| Mouse: KrasLSL-G12D
|
(Hingorani et al., 2003) |
Strain# JAX 008179 |
| Mouse: p53LoxP/LoxP
|
(Jonkers et al., 2001) |
Strain# JAX 008462 |
| Mouse: Rosa26LSL-Nsd2(E1099K)
|
This study |
N/A |
| Mouse: H11LSL-dCas9-KRAB-MeCP2; CAG-rtTA
|
This study |
N/A |
| Mouse: NOD.SCID-IL2Rg−/− (NSG) |
The Jackson Laboratories |
Strain# 005557 |
| Oligonucleotides |
| sgRNA non-targeting (control) 5’-CTTCGAAATGTCCGTTCGGT-3’ |
This study |
N/A |
| sgRNA Nsd2 human 5’- ACTCGTTAACAAATTCTCCCTGG 3’ |
This study |
N/A |
| sgRNA dCas9-KM Ci-Nsd2 5’- CGTTGCAGCGTAGGTCACTG-3’ |
This study |
N/A |
| sgRNA dCas9-KM Ci-Control 5’-GCGAGGTATTCGGCTCCGCG-3’ |
This study |
N/A |
| RT-qPCR Fosl1 forward 5’-CTAAGTGCAGAAACCGAAGAAAG 3’ |
This study |
N/A |
| RT-qPCR Fosl1 reverse 5’- CTTCTGCAGCTCTTCAATCTCTC-3’ |
This study |
N/A |
| RT-qPCR Jun forward 5’- TGGGCACATCA CCACTACAC-3’ |
This study |
N/A |
| RT-qPCR Jun reverse 5’- TCTGGCTATGCAGTTCAGCC -3’ |
This study |
N/A |
| RT-qPCR Actb forward 5’-TCGTACCACAGGCATTGTGATGG-3’ |
This study |
N/A |
| RT-qPCR Actb reverse 5’- GCAATGCCTGGGTACATGGTGG-3’ |
This study |
N/A |
| RT-qPCR Kras forward 5’- GGAGTACAGTGCAATGAGGGAC-3’ |
This study |
N/A |
| RT-qPCR Kras reverse 5’- CCAGGACCATAGGCACATCTTC-3’ |
This study |
N/A |
| ChIP-qPCR Fosl1 forward 5’- CCCCCGTGGTGCAAGTGGTT-3’ |
This study |
N/A |
| ChIP-qPCR Fosl1 reverse 5’- CGCGCCTCTCGGAGTCTGGT -3’ |
This study |
N/A |
| ChIP-qPCR Jun forward 5’- GCCATCCACAAATCCTCCCTGA -3’ |
This study |
N/A |
| ChIP-qPCR Jun reverse 5’- GCACAAGTGGGAAGTAACCCTA -3’ |
This study |
N/A |
| ChIP-qPCR GAPDH forward 5’- TAAGTCCCACAGCACCACAT -3’ |
This study |
N/A |
| ChIP-qPCR GAPDH reverse 5’- AGCATCTTTGTGACTGGGGA -3’ |
This study |
N/A |
| Recombinant DNA |
| Plasmid: pLentiCRISPRv2 |
Feng Zhang Lab |
Cat# Addgene #52961 |
| Plasmid: pCMV-dR8.2 dvpr |
Bob Weinberg Lab |
Cat# Addgene #8455 |
| Plasmid: pCMV-VSV-G |
Bob Weinberg Lab |
Cat# Addgene #8454 |
| Plasmid: pBABE-neo |
Bob Weinberg Lab |
Cat# Addgene #1767 |
| Plasmid: pGEX-6P-1 |
GE Healthcare |
Cat# 28-9546-48 |
| Plasmid: pENTR3C |
Thermo Fisher Scientific |
Cat# A10465 |
| Plasmid: pLenti CMV Hygro DEST (w117-1) |
Campeau and Kaufman lab |
Cat# Addgene 17454 |
| Software and Algorithms |
| Prism 7 |
GraphPad |
https://www.graphpad.com/; RRID:SCR_002798 |
| Excel for Mac 2016 |
Microsoft |
https://www.microsoft.com/en-us/; RRID:SCR_016137 |
| PreciPoint M8 ViewPoint |
PreciPoint |
http://www.precipoint.com/microscopy-software/viewpoint/
|
| ImageJ – Fiji package |
Freeware |
http://fiji.sc; RRID:SCR_002285 |
| Origin Pro 8 |
Microcal |
https://www.originlab.com/ RRID:SCR_002815 |
| DeepTools (3.5.0) |
(Ramirez et al., 2016) |
https://deeptools.readthedocs.io/en/latest/content/list_of_tools.html
|
| Samtools (1.9) |
(Li et al., 2009) |
http://www.htslib.org/doc/samtools.html
|
| Bamtools (2.3.0) |
(Barnett et al., 2011) |
https://github.com/pezmaster31/bamtools
|
| Picard (1.96) |
Picard Toolkit, 2019. Broad Institute, GitHub Repository |
https://broadinstitute.github.io/picard/
|
| FeatureCounts |
(Liao et al., 2014) |
http://subread.sourceforge.net
|
| Gene Set Enrichment Analysis (GSEA) (4.1.0) |
(Subramanian et al., 2005) |
https://www.gsea-msigdb.org/gsea/index.jsp
|
| Bowtie2 (2.2.7) |
(Langmead and Salzberg, 2012) |
http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
|
| HTSeq |
(Anders et al., 2015) |
https://htseq.readthedocs.io/en/release_0.11.1/count.html
|
| HISAT2 (2.2.1) |
(Kim et al., 2015) |
https://daehwankimlab.github.io/hisat2
|
| DESeq2 |
(Love et al., 2014) |
(https://bioconductor.org/packages/release/bioc/html/DESeq2.html
|
| Trim_galore (0.6.5) |
Babraham Bioinformatics |
http://www.bioinformatics.babraham.ac.uk/projects/trim_galore
|
