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. 2021 Oct 22;11:760787. doi: 10.3389/fonc.2021.760787

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Copyright © 2021 Charfi, Demeule, Currie, Larocque, Zgheib, Danalache, Ouanouki, Béliveau, Marsolais and Annabi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression of SORT1, CD133 and MMP-9 during in vitro vasculogenic mimicry. (A) ES-2 ovarian cancer and MDA-MB-231 TNBC-derived cells were seeded ontop of Matrigel and in vitro VM assessed for up to 24 h. Scale bar = 1000 μm. (B) Total RNA was isolated from ES-2 and MDA-MB-231 cells at initial (t = 0 h), at early maturation (t = 6 h) and complete maturation (t = 24 h) of 3D capillary-like structures. Gene expression levels of SORT1, CD133, and MMP-9 were assessed in triplicate by RT-qPCR from three independent experiments as described in the Methods sections.