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. 2021 Oct 22;11:760787. doi: 10.3389/fonc.2021.760787

Figure 4.

Figure 4

Effect of sortilin gene silencing on in vitro vasculogenic mimicry. Cells were transiently transfected with either scrambled siRNA (siScr) or specific sortilin siRNA (siSortilin) as described in the Methods section. The extent of sortilin silencing was assessed in ES-2 and MDA-MB-231 cells at (A) the protein level by immunoblotting of cell lysates, and at (B) the gene expression levels by RT-qPCR from total RNA. MMP-9 gene expression was also assessed in both cell models transfected with either siScr or specific siSortilin. Transfected cells were then seeded ontop of Matrigel for 12 h (ES-2 cells), or 24 h (MDA-MB-231 cells). Quantitation of total number of loops was performed for (C) ES-2 cells and (D) MDA-MB-231 cells as described in the Methods section. Quantitation performed in (A–D) results from 3 independent experiments. Scale bar = 1000 μm.