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. 2021 Oct 22;11:760787. doi: 10.3389/fonc.2021.760787

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Copyright © 2021 Charfi, Demeule, Currie, Larocque, Zgheib, Danalache, Ouanouki, Béliveau, Marsolais and Annabi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of TNBC-derived MDA-MB-231 cells in vitro vasculogenic mimicry by an anti-SORT1 antibody directed against the SORT1 extracellular domain. (A) MDA-MB-231 cells were seeded ontop of Matrigel in the presence of 25 nM of either mouse IgG1, rabbit IgG, anti-SORT1 mouse mAb, or anti-SORT1 rabbit pAb. Pictures were taken after 24 h of 3D capillary-like structures formation. The number of loops (blue) and branching (red) formed by the cells were quantified as described in the Methods section. Scale bar = 1000 μm. (B) Quantification of total number of loops. (n=3 ± SD).