Figure 3. Got2 deficiency decreases aspartate levels and HSC function.

(A) Western blots with antibodies against GOT2 and Histone H3 were performed using bone marrow cells from Mx1-cre; Got2^fl/fl and control wild-type mice. (B and C) Mx1-cre; Got2^fl/fl and control mice did not differ in size or appearance. (D) White blood cell, red blood cell, and platelet counts in the blood of Mx1-cre; Got2^fl/fl and control mice. (E) Cellularity of the bone marrow from one femur and one tibia, the spleen, and the thymus. (F and G) Aspartate (F) and glutamate (G) levels in HSCs/MPPs, HPCs, LK cells, and CD45⁺ cells from Mx1-cre; Got2^fl/fl and control bone marrow. (H-J) Numbers of HSCs (H), restricted hematopoietic progenitors (I), and differentiated hematopoietic cells (J) in the bone marrow from one femur and one tibia. (K and L) Numbers and sizes of colonies formed by 10,000 bone marrow cells. (M) Donor-derived CD45⁺, myeloid, B, and T cells in the blood of mice competitively transplanted with Mx1-cre; Got2^fl/fl or control donor bone marrow cells (n=25 recipient mice total from 5 independent experiments with 5 donors per genotype). (N) Cellularity of the bone marrow from one femur and one tibia, the spleen, and the thymus of Mx1-cre; Got1^fl/fl; Got2^fl/fl or control wild-type mice. (O-Q) Numbers of HSCs (O), restricted hematopoietic progenitors (P), and differentiated hematopoietic cells (Q) in the bone marrow from one femur and one tibia. (R) Donor-derived CD45⁺, myeloid, B, and T cells in the blood of mice competitively transplanted with Mx1-cre; Got1^fl/fl; Got2^fI/fI or control donor bone marrow cells (n=15 recipient mice total from 3 independent experiments with 3 donors per genotype). All data represent mean ± standard deviation (* P< 0.05; ** P<0.01; *** P<0.001). The number of mice analyzed per genotype is shown in each panel. See also Figure S3, Tables S1 and S3.