Sex-specific differences in C14:0- and C16:0- liver ceramide concentrations and their response to dietary FA. Effect of FA supplementation on C14:0- and C16:0- ceramide concentrations in WT and CerS6 KO mice (a) and comparison of these ceramide species in male and female mice (b). Data are shown as mean ± SEM, n=5. Checkered bars, FD diet; solid bars, Control diet; striped bars, FS diet. WT shown in black and KO shown in grey. *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001, determined by One-way ANOVA with Sidak’s multiple comparisons test.