FA over-supplementation increased very-long-chain sphingomyelin concentrations in WT and CerS6 KO mice of both sexes. Data presented as mean ± SEM, n=5. Checkered bars, FD diet; solid bars, Control diet; striped bars, FS diet. WT shown in black and KO shown in grey. *, p<0.05; **, p<0.01; ***, p<0.001; ****. p<0.0001, determined by One-way ANOVA with Sidak’s multiple comparisons test.