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. Author manuscript; available in PMC: 2022 Nov 4.
Published in final edited form as: Mol Cell. 2021 Aug 17;81(21):4527–4539.e8. doi: 10.1016/j.molcel.2021.08.007

Figure 6. The variable NTD is the major determinant of SidJ and SdjA specificity for the SidE effectors.

Figure 6.

(A) Cartoon representations of SidJSdjA-NTD (left) and SdjASidJ-HTH (right) chimeras depicting the interchanged regions, presented on a crystal structure of SidJ (PDBID: 6OQQ). Portions from SidJ are in green and SdjA in salmon.

(B) Glutamylation activity of SidJ59–851, SdjA36–789, SdjASidJ-HTH and SidJSdjA-NTD using full length SdeA (A), SdeB (B), SdeC (C) and SidE (E) as substrates. Reaction products were analyzed as in Figure 2D. Compare lanes 1 and 13 (SidJ loss of activity), 5 and 9 (SdjA gain of activity).