Table 1.
Data collection and refinement statistics.
| SidJ:CaM:SdeA (EMD-23862) (PDBID: 7MIR) | SidJ:CaM:SdeC (EMD-23863) (PDBID: 7MIS) | |
|---|---|---|
| Data collection and processing | ||
| Magnification | 81,000 | 81,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e−/Å−2) | 50 | 50 |
| Defocus range (μm) | 0.8 – 2.5 | 0.8 – 2.5 |
| Pixel size (Å) | 1.08 | 1.08 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 3,193,233 | 4,454,035 |
| Final particle images (no.) | 310,154 | 152,589 |
| Map resolution (Å) | 2.5 | 2.8 |
| FSC threshold | 0.143 | 0.143 |
| Refinement | ||
| Initial model used (PDB code) | 5YIM, 6S5T | SidJ-SdeA |
| Model resolution (Å) | 2.6 | 2.9 |
| FSC threshold | 0.5 | 0.5 |
| Map sharpening B factor (Å−2) | −37 | −47 |
| Model composition | ||
| Nonhydrogen atoms | 12,997 | 13,039 |
| Protein residues | 1,603 | 1,616 |
| Ligands | 5 | 6 |
| B factors (Å−2) | ||
| Protein | 48.5 | 54.9 |
| Ligands | 36.9 | 30.8 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.003 | 0.003 |
| Bond angles (°) | 0.485 | 0.468 |
| Validation | ||
| MolProbity score | 1.27 | 1.42 |
| Clashscore | 2.83 | 3.74 |
| Poor rotamers (%) | 0 | 0 |
| Ramachandran plot | ||
| Favored (%) | 96.8 | 96.2 |
| Allowed (%) | 3.2 | 3.8 |
| Disallowed (%) | 0 | 0 |