KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-SidJ	(Black et al., 2019)	N/A
Rabbit anti-SdjA	This study	N/A
Rabbit anti-SdeA	(Black et al., 2019)	N/A
Mouse anti-GAPDH	ThermoFisher	Cat#MA5-15738
Mouse anti-HA antibody	Sigma-Aldrich	Cat# H3663
Donkey anti-Mouse IgG, HRP-Linked Whole Ab	VWR	Cat#95017-554
Donkey anti-Rabbit IgG, HRP-Linked Whole Ab	VWR	Cat# 95017-556
Bacterial Strains
Rosetta DE3	Novagen	Cat#70954
Rosetta DH5α	This study	N/A
Chemicals, Peptides, and Recombinant Proteins
L. pneumophila SidJ 59-851 (E. coli)	This study	N/A
L. pneumophila SidJ 97-851 (E. coli)	This study	N/A
L. pneumophila SdjA (E. coli)	This study	N/A
L. pneumophila SdjA 36-789 (E. coli)	This study	N/A
L. pneumophila SdeA (E. coli)	This study	N/A
L. pneumophila SdeB (E. coli)	This study	N/A
L. pneumophila SdeC (E. coli)	This study	N/A
L. pneumophila SidE (E. coli)	This study	N/A
L. pneumophila SdeA 231-1190 (E. coli)	This study	N/A
L. pneumophila SdeC 231-1222 (E. coli)	This study	N/A
Human CALM2 (E. coli)	This study	N/A
Human HA-Ubb (E. coli)	This study	N/A
Ulp1 S. cerevisiae SUMO protease	This study	N/A
PfuTurbo DNA Polymerase	Thermo	Cat# 50-125-946
Q5 High-Fidelity 2X Master Mix	NEB	M0492L
Adenosine 5’-triphosphate disodium salt hydrate	Sigma	Cat# A2383
ATP, [α-32P]- 3000Ci/mmol 10mCi/ml, 250 μCi	PerkinElmer	BLU003H250UC
L-[14C(U)]-Glutamic Acid	PerkinElmer	NEC290E050UC
L-[3,4-3H]-Glutamic Acid	PerkinElmer	NET490001MC
Budget-Solve scintillation cocktail	RPI	Cat# 111167
EN3HANCE Autoradiography Enhancer	PerkinElmer	Cat# 6NE9701
Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)	Fisher Scientific	Cat# PI20490
Hydrogen peroxide	Sigma	Cat# H1009
L-glutamic acid, Sodium salt	Sigma	Cat# G1626
Sodium Chloride	Fisher Scientific	BP358-10
Tris base	Fisher Scientific	BP152-5
Kanamycin sulfate	Millipore Sigma	K1377
Ampicillin sodium salt	Millipore Sigma	A9518
Chloramphenicol	Millipore Sigma	C0378
PolyJet In Vitro DNA Transfection Reagent	SignaGen Laboratories	SL100688
Protease Inhibitor Cocktail Tablets (Complete)	Millipore Sigma	11697498001
Dithiothreitol (DTT)	Millipore Sigma	D0632
DMEM, High Glucose	Thermo Fisher Scientific	11-965-118
Fetal bovine serum	Millipore Sigma	F2442
Penicillin-Streptomycin	Millipore Sigma	P0781
Magnesium Chloride hexahydrate	Millipore Sigma	M2670
Deposited Data
SidJ:CALM2:SdeA	This study	EMDB: EMD-23862 PDB: 7MIR
SidJ:CALM2:SdeC	This study	EMDB: EMD-23863 PDB: 7MIS
Experimental Models: Organisms/Strains
Legionella pneumophila Philadelphia-1 (Lp02)	Dr. Ralph Isberg	N/A
Legionella pneumophila Philadelphia-1 ΔdotA (Lp03)	Dr. Ralph Isberg	N/A
Lp02 ΔsidJ	(Black et al., 2019)	N/A
Lp02 ΔsdjA	This study	N/A
Lp02 ΔsdeA	This study	N/A
Lp02 ΔsidJΔsdjA	This study	N/A
Lp02 ΔsidJΔsdjA (pJB908 sidJ)	This study	N/A
Lp02 ΔsidJΔsdjA (pJB908 sidJ D542A)	This study	N/A
Lp02 ΔsidJΔsdjA (pJB908 sdjA)	This study	N/A
Lp02 ΔsidJΔsdjA (pJB908 sdjA D480A)	This study	N/A
Lp02 ΔsidJΔsdjA (pJB908)	This study	N/A
Lp02 ΔsidJΔsdjAΔsdeA	This study	N/A
Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sidJ)	This study	N/A
Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sidJ D542A)	This study	N/A
Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sdjA)	This study	N/A
Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sdjA D480A)	This study	N/A
Lp02 ΔsidJΔsdjAΔsdeA (pJB908)	This study	N/A
HEK293a	Invitrogen	R70507
Acanthamoeba castellanii	Dr. Kim Orth	N/A
Oligonucleotides
See Table S1 for primers used in this study
Recombinant DNA
ppSUMO L. pneumophila SidJ 59-851	(Black et al., 2019)	N/A
ppSUMO L. pneumophila SidJ 97-851	This study	N/A
ppSUMO L. pneumophila SdjA 36-789	This study	N/A
ppSUMO CALM2	(Black et al., 2019)
ppSUMO L. pneumophila SdeA	(Black et al., 2019)	N/A
ppSUMO L. pneumophila SdeB	(Black et al., 2019)	N/A
ppSUMO L. pneumophila SdeC	(Black et al., 2019)	N/A
ppSUMO L. pneumophila SidE	(Black et al., 2019)	N/A
ppSUMO L. pneumophila SdeA 231-1190	This study	N/A
ppSUMO L. pneumophila SdeC 231-1222	This study	N/A
ppSUMO L. pneumophila SidJSdjA-NTD	This study	N/A
ppSUMO L. pneumophila SdjASidJ-HTH	This study	N/A
pETDuet1 L. pneumophila SdjA CALM2	This study	N/A
pProEx2 L. pneumophila SdeA 231-1190	This study	N/A
pProEx2 L. pneumophila SdeC 231-1222	This study	N/A
pSR47s	Dr. Shaeri Mukherjee	N/A
pSR47s sdeA flanks	This study	N/A
pSR47s sdjA flanks	This study	N/A
pSR47s sidJ flanks	(Black et al., 2019)	N/A
pJB908 sidJ	(Black et al., 2019)	N/A
pJB908 sidJ D542A	(Black et al., 2019)	N/A
pJB908 SdjA	This study	N/A
pJB908 SdjA D480A	This study	N/A
pJB908	Dr. Ralph Isberg	N/A
pcDNA-HA-Ubb	Dr. Jenna Jewell	N/A
pcDNA-HA-Ubb GG/AA	(Black et al., 2019)	N/A
pcDNA-SidJ-V5	(Black et al., 2019)	N/A
pcDNA-SidJ-V5 D542A	(Black et al., 2019)	N/A
pCCF-V5-SdjA	This study	N/A
pCCF-V5-SdjA D480A	This study	N/A
pCDNA-Myc-SdeA (c. opt)	(Black et al., 2019)	N/A
pCCF-Myc-SdeB	(Black et al., 2019)	N/A
pCCF-Myc-SdeC (c. opt)	(Black et al., 2019)	N/A
pCCF-Myc-SidE	(Black et al., 2019)	N/A
Software and Algorithms
Pymol	Schrödinger, Inc.	N/A
WebLogo server	(Crooks et al., 2004)	http://weblogo.threeplusone.com/
Dalilite server	(Holm and Rosenstrom, 2010)	http://ekhidna2.biocenter.helsinki.fi/dali/
Jackhmmer server	(Finn et al., 2015)	https://www.ebi.ac.uk/Tools/hmmer/search/jackhmmer
BLAST server	(Altschul et al., 1990)	https://blast.ncbi.nlm.nih.gov/Blast.cgi
MAFFT server	(Katoh and Standley, 2013)	https://mafft.cbrc.jp/alignment/software/
Pfam database	(Finn et al., 2016)	http://pfam.xfam.org/
RELION	(Zivanov et al., 2018)	https://relion.readthedocs.io/en/latest/index.html
crYOLO	(Wagner et al., 2019)	https://cryolo.readthedocs.io/en/stable/
Gctf	(Zhang, 2016)	https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/
DeepEMhancer	(Sanchez-Garcia et al., 2020)	https://github.com/rsanchezgarc/deepEMhancer
Chimera	(Pettersen et al., 2004)	https://www.cgl.ucsf.edu/chimera/
Phenix	(Adams et al., 2010)	https://www.phenix-online.org
Coot	(Emsley et al., 2010)	https://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot
CCP4 7.1	(Winn et al., 2011)	https://www.ccp4.ac.uk/?page_id=1417
GraphPad Prism	N/A	https://www.graphpad.com/scientific-software/prism/
ESPript	(Robert and Gouet, 2014)	http://espript.ibcp.frESPript/ESPript/