KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-SidJ | (Black et al., 2019) | N/A |
| Rabbit anti-SdjA | This study | N/A |
| Rabbit anti-SdeA | (Black et al., 2019) | N/A |
| Mouse anti-GAPDH | ThermoFisher | Cat#MA5-15738 |
| Mouse anti-HA antibody | Sigma-Aldrich | Cat# H3663 |
| Donkey anti-Mouse IgG, HRP-Linked Whole Ab | VWR | Cat#95017-554 |
| Donkey anti-Rabbit IgG, HRP-Linked Whole Ab | VWR | Cat# 95017-556 |
| Bacterial Strains | ||
| Rosetta DE3 | Novagen | Cat#70954 |
| Rosetta DH5α | This study | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| L. pneumophila SidJ 59-851 (E. coli) | This study | N/A |
| L. pneumophila SidJ 97-851 (E. coli) | This study | N/A |
| L. pneumophila SdjA (E. coli) | This study | N/A |
| L. pneumophila SdjA 36-789 (E. coli) | This study | N/A |
| L. pneumophila SdeA (E. coli) | This study | N/A |
| L. pneumophila SdeB (E. coli) | This study | N/A |
| L. pneumophila SdeC (E. coli) | This study | N/A |
| L. pneumophila SidE (E. coli) | This study | N/A |
| L. pneumophila SdeA 231-1190 (E. coli) | This study | N/A |
| L. pneumophila SdeC 231-1222 (E. coli) | This study | N/A |
| Human CALM2 (E. coli) | This study | N/A |
| Human HA-Ubb (E. coli) | This study | N/A |
| Ulp1 S. cerevisiae SUMO protease | This study | N/A |
| PfuTurbo DNA Polymerase | Thermo | Cat# 50-125-946 |
| Q5 High-Fidelity 2X Master Mix | NEB | M0492L |
| Adenosine 5’-triphosphate disodium salt hydrate | Sigma | Cat# A2383 |
| ATP, [α-32P]- 3000Ci/mmol 10mCi/ml, 250 μCi | PerkinElmer | BLU003H250UC |
| L-[14C(U)]-Glutamic Acid | PerkinElmer | NEC290E050UC |
| L-[3,4-3H]-Glutamic Acid | PerkinElmer | NET490001MC |
| Budget-Solve scintillation cocktail | RPI | Cat# 111167 |
| EN3HANCE Autoradiography Enhancer | PerkinElmer | Cat# 6NE9701 |
| Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP) | Fisher Scientific | Cat# PI20490 |
| Hydrogen peroxide | Sigma | Cat# H1009 |
| L-glutamic acid, Sodium salt | Sigma | Cat# G1626 |
| Sodium Chloride | Fisher Scientific | BP358-10 |
| Tris base | Fisher Scientific | BP152-5 |
| Kanamycin sulfate | Millipore Sigma | K1377 |
| Ampicillin sodium salt | Millipore Sigma | A9518 |
| Chloramphenicol | Millipore Sigma | C0378 |
| PolyJet In Vitro DNA Transfection Reagent | SignaGen Laboratories | SL100688 |
| Protease Inhibitor Cocktail Tablets (Complete) | Millipore Sigma | 11697498001 |
| Dithiothreitol (DTT) | Millipore Sigma | D0632 |
| DMEM, High Glucose | Thermo Fisher Scientific | 11-965-118 |
| Fetal bovine serum | Millipore Sigma | F2442 |
| Penicillin-Streptomycin | Millipore Sigma | P0781 |
| Magnesium Chloride hexahydrate | Millipore Sigma | M2670 |
| Deposited Data | ||
| SidJ:CALM2:SdeA | This study | EMDB: EMD-23862 PDB: 7MIR |
| SidJ:CALM2:SdeC | This study | EMDB: EMD-23863 PDB: 7MIS |
| Experimental Models: Organisms/Strains | ||
| Legionella pneumophila Philadelphia-1 (Lp02) | Dr. Ralph Isberg | N/A |
| Legionella pneumophila Philadelphia-1 ΔdotA (Lp03) | Dr. Ralph Isberg | N/A |
| Lp02 ΔsidJ | (Black et al., 2019) | N/A |
| Lp02 ΔsdjA | This study | N/A |
| Lp02 ΔsdeA | This study | N/A |
| Lp02 ΔsidJΔsdjA | This study | N/A |
| Lp02 ΔsidJΔsdjA (pJB908 sidJ) | This study | N/A |
| Lp02 ΔsidJΔsdjA (pJB908 sidJ D542A) | This study | N/A |
| Lp02 ΔsidJΔsdjA (pJB908 sdjA) | This study | N/A |
| Lp02 ΔsidJΔsdjA (pJB908 sdjA D480A) | This study | N/A |
| Lp02 ΔsidJΔsdjA (pJB908) | This study | N/A |
| Lp02 ΔsidJΔsdjAΔsdeA | This study | N/A |
| Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sidJ) | This study | N/A |
| Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sidJ D542A) | This study | N/A |
| Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sdjA) | This study | N/A |
| Lp02 ΔsidJΔsdjAΔsdeA (pJB908 sdjA D480A) | This study | N/A |
| Lp02 ΔsidJΔsdjAΔsdeA (pJB908) | This study | N/A |
| HEK293a | Invitrogen | R70507 |
| Acanthamoeba castellanii | Dr. Kim Orth | N/A |
| Oligonucleotides | ||
| See Table S1 for primers used in this study | ||
| Recombinant DNA | ||
| ppSUMO L. pneumophila SidJ 59-851 | (Black et al., 2019) | N/A |
| ppSUMO L. pneumophila SidJ 97-851 | This study | N/A |
| ppSUMO L. pneumophila SdjA 36-789 | This study | N/A |
| ppSUMO CALM2 | (Black et al., 2019) | |
| ppSUMO L. pneumophila SdeA | (Black et al., 2019) | N/A |
| ppSUMO L. pneumophila SdeB | (Black et al., 2019) | N/A |
| ppSUMO L. pneumophila SdeC | (Black et al., 2019) | N/A |
| ppSUMO L. pneumophila SidE | (Black et al., 2019) | N/A |
| ppSUMO L. pneumophila SdeA 231-1190 | This study | N/A |
| ppSUMO L. pneumophila SdeC 231-1222 | This study | N/A |
| ppSUMO L. pneumophila SidJSdjA-NTD | This study | N/A |
| ppSUMO L. pneumophila SdjASidJ-HTH | This study | N/A |
| pETDuet1 L. pneumophila SdjA CALM2 | This study | N/A |
| pProEx2 L. pneumophila SdeA 231-1190 | This study | N/A |
| pProEx2 L. pneumophila SdeC 231-1222 | This study | N/A |
| pSR47s | Dr. Shaeri Mukherjee | N/A |
| pSR47s sdeA flanks | This study | N/A |
| pSR47s sdjA flanks | This study | N/A |
| pSR47s sidJ flanks | (Black et al., 2019) | N/A |
| pJB908 sidJ | (Black et al., 2019) | N/A |
| pJB908 sidJ D542A | (Black et al., 2019) | N/A |
| pJB908 SdjA | This study | N/A |
| pJB908 SdjA D480A | This study | N/A |
| pJB908 | Dr. Ralph Isberg | N/A |
| pcDNA-HA-Ubb | Dr. Jenna Jewell | N/A |
| pcDNA-HA-Ubb GG/AA | (Black et al., 2019) | N/A |
| pcDNA-SidJ-V5 | (Black et al., 2019) | N/A |
| pcDNA-SidJ-V5 D542A | (Black et al., 2019) | N/A |
| pCCF-V5-SdjA | This study | N/A |
| pCCF-V5-SdjA D480A | This study | N/A |
| pCDNA-Myc-SdeA (c. opt) | (Black et al., 2019) | N/A |
| pCCF-Myc-SdeB | (Black et al., 2019) | N/A |
| pCCF-Myc-SdeC (c. opt) | (Black et al., 2019) | N/A |
| pCCF-Myc-SidE | (Black et al., 2019) | N/A |
| Software and Algorithms | ||
| Pymol | Schrödinger, Inc. | N/A |
| WebLogo server | (Crooks et al., 2004) | http://weblogo.threeplusone.com/ |
| Dalilite server | (Holm and Rosenstrom, 2010) | http://ekhidna2.biocenter.helsinki.fi/dali/ |
| Jackhmmer server | (Finn et al., 2015) | https://www.ebi.ac.uk/Tools/hmmer/search/jackhmmer |
| BLAST server | (Altschul et al., 1990) | https://blast.ncbi.nlm.nih.gov/Blast.cgi |
| MAFFT server | (Katoh and Standley, 2013) | https://mafft.cbrc.jp/alignment/software/ |
| Pfam database | (Finn et al., 2016) | http://pfam.xfam.org/ |
| RELION | (Zivanov et al., 2018) | https://relion.readthedocs.io/en/latest/index.html |
| crYOLO | (Wagner et al., 2019) | https://cryolo.readthedocs.io/en/stable/ |
| Gctf | (Zhang, 2016) | https://www2.mrc-lmb.cam.ac.uk/research/locally-developed-software/zhang-software/ |
| DeepEMhancer | (Sanchez-Garcia et al., 2020) | https://github.com/rsanchezgarc/deepEMhancer |
| Chimera | (Pettersen et al., 2004) | https://www.cgl.ucsf.edu/chimera/ |
| Phenix | (Adams et al., 2010) | https://www.phenix-online.org |
| Coot | (Emsley et al., 2010) | https://www2.mrc-lmb.cam.ac.uk/Personal/pemsley/coot |
| CCP4 7.1 | (Winn et al., 2011) | https://www.ccp4.ac.uk/?page_id=1417 |
| GraphPad Prism | N/A | https://www.graphpad.com/scientific-software/prism/ |
| ESPript | (Robert and Gouet, 2014) | http://espript.ibcp.frESPript/ESPript/ |