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. 2021 Oct 9;297(5):101295. doi: 10.1016/j.jbc.2021.101295

Table 1.

Kinetic parameters of the interaction between the cytokine and the respective receptor

Cytokine Receptor kon (M−1 s−1) koff (s−1) KD (nM)
HIL-23 IL-23R 3.0 × 104 1.2 × 10−3 40
HIL-23D36K/W37K/T38K IL-23R 1.9 × 104 1.1 × 10−3 57
HIL-12 IL-12Rβ2 1.9 × 106 1.9 × 10−3 0.987
HIL-12D36K/W37K/T38K IL-12Rβ2 3.2 × 106 2.7 × 10−3 0.840

SPR analysis of HIL-23 and HIL-12 variants binding to IL-23R-Fc and IL-12Rβ2-Fc. IL-23R-Fc and IL-12Rβ2-Fc were immobilized on a Protein A chip and increasing concentrations of HIL-23 or HIL-12 variants were injected at a flow rate of 30 μl/min. Measurements were carried out on a Biacore X100 instrument.