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Molecular information of Mint3.A, Mint3 constructs used in this study. B, CD spectrum of Mint3NT. C, HDX-MS chart of Mint3NT. D and E, comparison of the 1H-15N HSQC spectra of Mint3NT (D) and the superposition (E) of the fragment proteins of Mint3NT, corresponding to the N-terminal half (Mint3(1–117) in blue) and the C-terminal half (Mint3(101–214) in red). All spectra were recorded under nearly physiological conditions at pH 7.3 and 4 °C.
