Confirmation of the role of novel binding in cells.A, immunoblotting using mouse anti-V5 antibody and rabbit anti-FLAG polyclonal antibody in 293FT cells. Left side is a sample of the whole cell lysate, while right side is the sample immunoprecipitated using an anti-FLAG polyclonal antibody. B, reporter assay expressed vector alone, wild-type Mint3, or Mint3mut2 in 293FT cells. Left side is the immunoblotting data of the whole cell lysate using an anti-V5 antibody, while right side is the data for the luciferase activities.