Table 2.
Frequency of top 5 intratumoral CD8+ T cell clones in the spleen
| Mouse | CDR3β sequence | % in tumor | % in spleen |
|---|---|---|---|
| PBS-1 | CGARQSNTEVFF | 3.93 | 0.00 |
| CASSLDWGDYEQYF | 3.02 | 0.00 | |
| CASRQGEQYF | 2.91 | 0.00 | |
| CASSQVLGDTQYF | 2.76 | 0.00 | |
| CASSPDWVYAEQFF | 2.50 | 0.00 | |
| PBS-2 | CASSLELGGPTQYF | 14.41 | 0.00 |
| CASSQEQTINYAEQFF | 3.15 | 0.00 | |
| CASSIKVANTEVFF | 2.29 | 0.00 | |
| CASSLLGAYEQYF | 2.26 | 0.02 | |
| CASSPTGGNYAEQFF | 2.10 | 0.00 | |
| PBS-3 | CASSLRVAEVFF | 34.33 | 0.00 |
| CASSLEGTAETLYF | 25.68 | 0.00 | |
| CASSIRGYEQYF | 13.78 | 0.00 | |
| CASSLVGGASYEQYF | 12.02 | 0.00 | |
| CGAGTGETEQFF | 4.57 | 0.00 | |
| PBS-4 | CTCSADGAGGGNERLFF | 6.34 | 0.00 |
| CASSFSFSAETLYF | 5.89 | 0.00 | |
| CASSQTGEHTQYF | 4.22 | 0.00 | |
| CASSLSGSDYTF | 3.73 | 0.00 | |
| CASSFRGGQNTLYF | 3.01 | 0.00 | |
| PBS-5 | CASSLELGGPEQYF | 7.74 | 0.00 |
| CASSDPGGSAETLYF | 3.77 | 0.00 | |
| CASSLGTKDTQYF | 3.43 | 0.00 | |
| CASRDRGTEVFF | 3.03 | 0.00 | |
| CASGDALGVYEQYF | 2.63 | 0.00 | |
| Cont-VV-1 | CASSLRDKKDTQYF | 5.78 | 0.01 |
| CASSFTGTNNQAPLF | 5.69 | 0.08 | |
| CASSLRSQNTLYF | 5.61 | 0.00 | |
| CASSGRDRVSAEQFF | 3.08 | 0.00 | |
| CASSNTGYNNQAPLF | 2.47 | 0.03 | |
| Cont-VV-2 | CASSFTGRNNQAPLF | 18.66 | 0.44 |
| CASSPRDRDGNTLYF | 6.72 | 0.02 | |
| CASSLPGQNTEVFF | 3.55 | 0.00 | |
| CASSLGTGSYEQYF | 2.82 | 0.00 | |
| CASSDAGQGAEVFF | 2.28 | 0.00 | |
| Cont-VV-3 | CASSLHSAETLYF | 5.87 | 0.57 |
| CASSSTGYNNQAPLF | 5.86 | 0.59 | |
| CASSPGTGRNNQAPLF | 3.40 | 0.15 | |
| CASSRTGDSYEQYF | 2.26 | 0.00 | |
| CASSLVPAETLYF | 1.90 | 0.07 | |
| Cont-VV-4 | CASRGQISNERLFF | 6.82 | 0.00 |
| CASSLGLGAYEQYF | 3.78 | 0.00 | |
| CASSIRGGRGAETLYF | 3.31 | 0.00 | |
| CASSSTGHNNQAPLF | 3.18 | 0.01 | |
| CTCSVDRVDTGQLYF | 3.02 | 0.00 | |
| Cont-VV-5 | CASSSTGENNQAPLF | 7.24 | 0.02 |
| CAWSQQGRNNQAPLF | 6.89 | 0.00 | |
| CASGGWGGQNTLYF | 6.08 | 0.00 | |
| CASSLLDWGSYAEQFF | 6.04 | 0.02 | |
| CASSKTGGDTQYF | 5.54 | 0.00 | |
| hIL-7-VV-1 | CASSLGTTNERLFF | 7.92 | 0.00 |
| CASSLGHQNTLYF | 6.00 | 0.00 | |
| CASSPNWGGQDTQYF | 4.57 | 0.20 | |
| CASSHQDTEVFF | 4.18 | 0.00 | |
| CASSLFDRAYAEQFF | 2.98 | 0.03 | |
| hIL-7-VV-2 | CASSQPGAYEQYF | 4.78 | 0.00 |
| CASGRDFYEQYF | 3.50 | 0.00 | |
| CASSPGTYEQYF | 2.89 | 0.00 | |
| CASSRQGENNSPLYF | 2.75 | 0.00 | |
| CASSSTGYNNQAPLF | 2.47 | 0.02 | |
| hIL-7-VV-3 | CASSLRDWGAYAEQFF | 4.85 | 0.00 |
| CASSPGLGEGEQFF | 4.63 | 0.00 | |
| CTCSADRQEDTQYF | 2.84 | 0.00 | |
| CASSPRQIQDTQYF | 2.55 | 0.01 | |
| CASSAGTAYEQYF | 2.49 | 0.00 | |
| hIL-7-VV-4 | CASSRQGENNQAPLF | 4.24 | 0.03 |
| CASSLSYEQYF | 3.58 | 0.00 | |
| CASGGPYEQYF | 3.58 | 0.00 | |
| CTCSAYRASQNTLYF | 3.47 | 0.00 | |
| CASSLAWGGRRNTLYF | 2.82 | 0.00 | |
| mIL-12-VV-1 | CASSPDWGGAETLYF | 9.65 | 0.06 |
| CASSSGWGRNYAEQFF | 9.25 | 0.26 | |
| CASSYRGLEQYF | 4.42 | 0.26 | |
| CASGEGYGGAQRNTLYF | 3.69 | 2.21 | |
| CASSLRQNSDYTF | 3.57 | 0.15 | |
| mIL-12-VV-2 | CASSIGDQDTQYF | 55.79 | 0.00 |
| CASSQGNYAEQFF | 28.25 | 0.85 | |
| CGARVRGNSDYTF | 10.98 | 0.44 | |
| CASSQGNYAGQFF | 0.15 | 0.00 | |
| CANSIGDQDTQYF | 0.14 | 0.00 | |
| mIL-12-VV-3 | CASRTANTEVFF | 36.27 | 3.29 |
| CASSLTTANTEVFF | 5.04 | 0.06 | |
| CASSYRDSDYTF | 2.85 | 0.18 | |
| CASTWGGNTLYF | 2.29 | 0.17 | |
| CASSPQGAETLYF | 2.27 | 0.00 | |
| mIL-12-VV-4 | CASSPDWGGAETLYF | 6.75 | 0.13 |
| CASSYGGASYEQYF | 5.39 | 0.08 | |
| CGARQNTEVFF | 4.76 | 1.00 | |
| CASSLGQTANERLFF | 1.75 | 0.08 | |
| CASSFLGGLEQYF | 1.52 | 0.49 | |
| hIL-7/mIL-12-VV-1 | CASSLTGGGQNTLYF | 12.13 | 0.08 |
| CASSQGQGSQNTLYF | 11.05 | 0.79 | |
| CASSTGGGYAEQFF | 8.14 | 0.08 | |
| CASSLELGGREQYF | 6.27 | 0.03 | |
| CASGDARLVSSYEQYF | 4.33 | 0.00 | |
| hIL-7/mIL-12-VV-2 | CASSVRDREDEQYF | 39.77 | 0.00 |
| CASSFSPSNERLFF | 25.90 | 0.04 | |
| CASSGTISNERLFF | 24.95 | 0.04 | |
| CASSTPGTGGYEQYF | 1.27 | 0.00 | |
| CASSFSPANERLFF | 0.23 | 0.00 | |
| hIL-7/mIL-12-VV-3 | CASSLGTGGEEQYF | 42.39 | 0.71 |
| CTCSEGWGEQNTLYF | 26.86 | 0.00 | |
| CASSLGVSQNTLYF | 24.04 | 0.00 | |
| RASSLGTGGEEQYF | 0.15 | 0.00 | |
| CASSVGTGGEEQYF | 0.15 | 0.00 | |
| hIL-7/mIL-12-VV-4 | CASSRQGAERLFF | 26.21 | 0.00 |
| CASSSGLGEDTGQLYF | 21.34 | 0.00 | |
| CASSSRDRGGETLYF | 8.66 | 0.00 | |
| CASSRDLVSSYEQYF | 5.21 | 0.00 | |
| CGAKLGVQDTQYF | 4.42 | 0.14 | |
| hIL-7/mIL-12-VV-5 | CASSPGTSSQNTLYF | 10.14 | 0.03 |
| CASSQTRDWGYEQYF | 5.51 | 0.57 | |
| CASSPNWGEGDTQYF | 3.87 | 0.06 | |
| CASSPPGGDEQYF | 3.65 | 0.19 | |
| CASSLLNYAEQFF | 2.51 | 0.21 |
Information about TRBV, TRBJ, the number of individuals reads, and the number of total in-frame reads in each sample are described in Supplementary Data File 1