Fig. 5.
Polyfunctionality in tumor-infiltrating MAIT cells. Single cell suspensions were isolated from colon tumors, and production of IFN-γ, GrB, IL-2, and TNF by MAIT cells evaluated in vitro by flow cytometry after polyclonal stimulation with PMA and Ionomycin. a Percentage of PD-1hiTim-3+ and PD-1−Tim-3− MAIT cells from individual patients expressing 4 (red), 3 (yellow), 2 (green), 1 (turquoise) or 0 (blue) of the analyzed effector molecules illustrated as pie charts. b Frequencies of PD-1hiTim 3+ (white bars) and PD-1−Tim 3− (grey bars) MAIT cells expressing 4, 3, 2, 1, or 0 of the analyzed effector molecules, shown as the percentage of total MAIT cells. c Polyfunctionality index for PD-1hiTim-3+ and PD-1−Tim-3− MAIT cells analyzed for 4 (GrB and cytokines) or 3 effector molecules (only cytokines). d Frequencies of PD-1hiTim-3+ (white bars) and PD-1−Tim-3− (grey bars) MAIT cell subsets expressing a combination of IFN-γ, GrB, IL-2, and TNF shown as the percentage of total MAIT cells. Symbols represent individual values and bars the mean. *p < 0.05 using Wilcoxon matched-pairs signed rank test. n = 7